阳离子交换树脂与大孔吸附树脂联用富集桑叶中总生物碱与总酚的工艺研究

Study on Enriching Total Alkaloids and Total Phenols from Mulberry by Cation Exchange Resin and Macroporous Absorption Resin

摘要: 目的富集桑叶中总生物碱和总酚。方法联合应用阳离子交换树脂和大孔吸附树脂对桑叶中总生物碱和总酚成分进行富集纯化，并对工艺进行优化和验证。结果该工艺可较好的富集纯化桑叶中总生物碱和总酚成分，指标性成分1-脱氧野尻霉素（DNJ）、绿原酸、芦丁的含量可达到0.17%、3.05%、0.92%。结论阳离子交换树脂与大孔吸附树脂联用富集桑叶中总生物碱与总酚的方法可行，适合于大生产。

Abstract: OBJECTIVE To enrich the total alkaloids and total phenols from mulberry. METHODS Combined appling cation exchange resin and macroporous absorption resin to enrich and purify the total alkaloids and total phenols， then verify and optimize the process. RESULTS The process contributed to enrich and purify the total alkaloids and total phenols from mulberry. The content of DNJ， chlorogenic acid and rutin can achieve 0.17%， 3.05% and 0.92%. CONCLUSION Using cation exchange resin and macroporous absorption resin to concentration of the total alkaloids and total phenols may be applied for the real bulk production.