C57/BL6小鼠母婴分离模型中医证型探讨
Investigation of TCM Syndromes on Maternal Separation in C57/BL6 Mice
-
摘要: 目的 探讨C57/BL6小鼠母婴分离（MS）模型中医证型。方法 通过母婴分离干预新生鼠，采用旷场实验评价母婴分离对小鼠自发活动的影响，强迫游泳和强迫悬尾实验评价母婴分离对小鼠不动时间的影响。四诊计量化指标检测与计量辨证方法探讨小鼠气血阴阳状态。通过促肾上腺皮质激素（ACTH）、皮质酮（CORT）测定，观察下丘脑-垂体-肾上腺皮质轴（HPA）功能状态。通过环磷酸腺苷/环磷酸鸟苷（cAMP/cGMP）、三磷酸腺苷酶（ATPase）及超氧化物歧化酶（SOD），探讨能量代谢水平。结果 旷场实验中各组动物活动总路径差异无统计学意义（P>0.05）。MS180组在强迫游泳实验、强迫悬尾实验中，与对照组及MS15组比较小鼠不动时间显著增加（P<0.01）。MS180组阳热水平较对照组和MS15组显著降低（P<0.01）。MS180组ACTH及CORT值升高，与对照组及MS15组比较有显著性差异（P<0.01）。MS180组cAMP/cGMP降低，与对照组和MS15组比较有显著性差异（P<0.01）。MS180组Na+-K+-ATPase活性降低，与对照组和MS15组比较有显著性差异（P<0.01）。MS180组SOD活性降低，与对照组和MS15组比较有显著性差异（P<0.01）。结论 母婴分离对小鼠行为有显著影响，小鼠出现抑郁样行为，小鼠HPA轴功能受到影响，机体能量代谢水平降低，小鼠表现出的证候特征属于中医阳虚证。Abstract: OBJECTIVE To investigate TCM syndromes on maternal separation in C57/BL6 mice. METHODS Neonatal mice were intervened through maternal separation. The effects of maternal separation on spontaneous activities of mice were evaluated by field test and the effects on immobility times of mice were evaluated by forced swimming test and tail suspension test. Four diagnostic quantitative indicators measurement and quantitative dialectical method were employed to explore the state of Qi， Blood， Yin and Yang in mice. Hypothalamus-pituitary-adrenal (HPA) cortical axis function was observed through adrenocorticotropic hormone (ACTH) and corticosterone (CORT) determination. The level of energy metabolism was investigated through the measurement of cyclic adenosine monophosphate/cyclic guanosine monophosphate (cAMP/cGMP)， ATPase and superoxide dismutase(SOD). RESULTS No statistical significance (P>0.05) was observed in the total path of animal activities between each group in field test. Compared with the control group and MS15 group， in the forced swimming test and tail suspension test， the immobility time of mice of MS18 group was significantly increased (P<0.01). The levels of Yang heat， cAMP/cGMP， Na+-K+-ATPase and SOD significantly decreased (P<0.01)， while ACTH and CORT significantly increased (P<0.01) of MS18 group， compared to the control group and MS15 group. CONCLUSION Maternal separation shows significant effects on behavior in mice. The mice exhibit depression-like behavior， upset HPA axis balance and reduce body energy metabolism， which characterized as Yang deficiency symptoms in TCM.
-
Keywords:
- maternal separation /
- depression /
- behavior /
- TCM syndrome
-
-
[1] Xue W， Zhou X， Yi N， et al. Yueju pill rapidly induces antidepressant-like effects and acutely enhances BDNF expression in mouse brain[J]. Evid Based Complement Alternat Med， 2013: 184367. [2] Biggio F， Pisu MG， Garau A， et al. Maternal separation attenuates the effect of adolescent social isolation on HPA axis responsiveness in adult rats[J]. Eur Neuropsychopharmacol， 2014， 24(7): 1152-1161. [3] Vetulani J. Early maternal separation: a rodent model of depression and a prevailing human condition[J]. Pharmacol Rep， 2013， 65(6): 1451-1461. [4] Porsolt RD， Le Pichon M， Jalfre M. Depression: a new animal model sensitive to antidepressant treatments[J]. Nature， 1977， 266(564): 730-732. [5] Steru L， Chermat R， Thierry B， et al. The tail suspension test: a new method for screening antidepressants in mice[J]. Psychopharmacol(Berl)， 1985， 85(3): 367-370. [6] 方肇勤，潘志强，卢文丽，等．大鼠、小鼠常见证候计量化辨证方法的建立及其评价[J]．中国中医基础医学杂志，2007， 13（7）：502-505． [7] Fang ZQ， Pan ZQ， Lu WL， et al. Establishment and evaluation of metrological methods of syndrome differentiation about common syndrome in rats and mice[J]. Chin J Basic Med Tradit Chin Med， 2007， 13(7): 502-505. [8] Zhang TY， Chrétien P， Meaney MJ， et al. Influence of naturally occurring variations in maternal care on prepulse inhibition of acoustic startle and the medial prefrontal cortical dopamine response to stress in adult rats[J]. J Neurosci， 2005， 25(6): 1493-1502. [9] 陈英华，欧阳轶强，孙琪，等．肾阳虚证动物模型规范化研究中诊断指标选择的初步探讨[J]．中国中医基础医学杂志，2003， 9（10）：26-30． [10] Chen YH， Ou Yang YQ， Sun Q， et al. A primary discussion of diagnosis indexes choosing in standardization study of animal model with kidney-yang deficiency[J]. Chin J Basic Med Tradit Chin Med， 2003， 9(10): 26-30. [11] 王琦，姚实林，董静，等．阳虚体质者内分泌及免疫功能变化[J]．中西医结合学报，2008， 6（12）：1226-1232． [12] Wang Q， Yao SL， Dong J， et al. Changes of endocrine and immune function in subjects of yang deficiency constitution[J]. J Intergr Med， 2008， 6(12): 1226-1232. [13] 岳广欣，张玲，卢贺起，等．温阳解郁法对母婴分离小鼠行为模式及HPA轴功能的影响[J]．中国中医基础医学杂志，2014（1）：42-46． [14] 〖JP2〗Yue GX， Zhang L， Lu HQ， et al. Effects of Warming Yang and relieving depress method to behavior pattern and function of HPA axis in maternal separation mice[J]. Chin J Basic Med in Tradit Chin Med， 2014(1): 42-46.〖JP〗 [15] 陈琳，戴建国，赵玉男，等．助阳宁神方改善应激致小鼠抑郁样行为的实验研究[J]．南京中医药大学学报，2012， 28（3）：252-255． [16] Chen L， Dai JG， Zhao YN， et al. Experimental research on zhuyang ningshen fangs effects on stress-induced depression-like behaviour of mice[J]. J Nanjing Univ Tradit Chin Med， 2012， 28(3): 252-255. [17] 〖JP2〗Pryce CR， Feldon J. Long-term neurobehavioural impact of the postnatal environment in rats: manipulations， effects and mediating mechanisms[J]. Neurosci Biobehav Rev， 2003， 27(1/2): 57-71.〖JP〗
计量
- 文章访问数: 1040
- HTML全文浏览量: 13
- PDF下载量: 1150
下载:
