青翘HPLC指纹图谱研究

The HPLC Fingerprint of Green Forsythiae Fructus

  • 摘要: 目的 建立青翘的HPLC指纹图谱。方法 色谱柱:Thermo C18 Column(4.6 mm×250 mm,5 μm);柱温:30 ℃;流动相:乙腈-水,梯度洗脱;流速:1.0 mL/min,检测波长:203 nm。结果 建立了青翘的HPLC指纹图谱,并标定了21个共有峰,指认了其中的3个共有峰。结论 此法简便、准确、快速,为全面科学评价青翘质量提供了可靠的分析方法。

     

    Abstract: OBJECTIVE To establish HPLC fingerprint map of Green Fructus forsythiae. METHODS Chromatographic Column: Thermo C18 (250 mm×4.6 mm, 5 μm column); Column temperature: 30 ℃; Moving phase: acetonitrile-water, solution in gradient elution; Flow rate: 1.0 mL/min; The UV-detection wavelength: 203 nm. RESULTS The HPLC fingerprint map of Green Fructus forsythia has been established, with 21 common peaks marked, 3 of which is identified. CONCLUSION The established method is simple, accurate and rapid, which has provided reliable method for comprehensive evaluation of the quality of Green Fructus forsythia.

     

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