Abstract: To study the effect of pyretilysis, conscious-sedation, and blood clotting of water buffalo horn, to search the substantial basic of baffulo born. METHOD Pyretolysis experiment, spontaneous movement experiment and blood clotting experiment were used to investigate the bioactivity of water baffulo horn powder, heating water-pheresis and colding water-pheresis. Column chromatography was used to isolate the water baffulo horn heating water-pheresis into three fractions, which were investigated the antipyretic effects. RESULT The experiments showed that water baffulo horn powder, heating water-pberesis and colding water-pberesis, could reduce the times of spontaneous movement, shorten the time of blood clotting time, and reduce the temperature of febrile rat, and the effect of heating water-pheresis was better than colding water-pheresis. The fractions of high-molecular and low-molecular could reduce the temperature of febrile rat, the effect of mid-molecular was inconspicuous. CONCLUSION Water baffulo horn had the effect on pyretolysis, sedation and blood clotting. The fractions of high-molecular and low-molecular had the effects on pyretolysis.