Abstract: OBJECTIVE To search for the substitutes for Comu Rhinoceri Asiatici and Comu Saigae Tataricae and make evaluations. METHOD HPLC method was used to analyze aminoethyisulfonic acid and cholesterol, and spectrophotometry was used to analyze aminohexose and free protein in eight kinds of homs. RESULT Analyses were made on aminoethyisulfonic acid, cholesterol, spectrophotometry, aminohexose and protein. CONCLUSION The method was accurate, speedy and sensitive, with good reproductivity. The result shows that aminoethyisulfonic acid might be one of the active substances in medicated horns, with similar constituents in the micromoleculs of different kinds of horns but different contents.