Abstract: OBJECTIVE To investigate into the effect of Ningxin Hongqi Capsule on the hormone level, pituitary gland, ovary and adrenal of female senile rats. METHOD SD female senile rats of 14 months old were divided randomly into a model group, a positive drug group, a Ninghong low dosage group, a Ninghong medium dosage group and a Ninghong high dosage group. SD female rats of 4 months old formed a youth group. Two months after drug administration, rat blood was taken and radioimmunoassay was employed to determine the levels of aquadiol, corporin, follicle stimulating hormone and luteinizing hormone. The coefficients of the pituitary gland, ovary and adrenal were eompared. RESULT Ningxin Hongqi Capsule could increase the decreased E2 level, decrease the increased FSH and LH level, increase the quality of the pituitary gland, ovary and adrenal. CONCLUSION Ningxin Hongqi Capsule can relieve the atrophy of the pituitary gland, ovary and adrenal and regulate the functions of pituitary gland and ovary axis.