HPLC测定北细辛不同部位及穴贴定喘膏中马兜铃酸A的含量

HPLC Determination of Content of Aristolochic Acid A in Different Parts of Herba cum Radice Asari and in Xuetie Dingchuan Plaster

摘要: 目的测定细辛不同药用部位及其制剂中马兜铃酸A的含量。方法采用HPLC方法,测定不同来源、不同批次的细辛药材;不同药用部位及其制剂定喘穴贴膏中马兜铃酸A的含量。结果细辛地上部分粗粉中马兜铃酸A含量高于细辛根茎粗粉中马兜铃酸A含量,制剂中没有检出马兜铃酸A。结论2005年版的《中国药典》将细辛药用部位由全草改为根茎是合理的,穴贴定喘膏未检出马兜铃酸A。

Abstract: OBJECTIVE To determine the content of Aristolochic Acid A in different parts of Herba cum Radice Asari and in Xuetie Dingchuan Plaster.METHOD HPLC method was used in the determination.RESULT The content of aristolochic acid A in the parts of Herba cum Radice Asari above the ground was higher that in the root part,and aristolochic acid A was not detected in the preparation.CONCLUSION It is reasonable that the 2005 edition of Chinese Pharmacopoeia specifies that the medicinal part of Herba cum Radice Asari is the root part,not the whole plant.Aristolochic acid A was not detected in Xuetie Dingchuan Plaster.