Abstract: OBJECTIVE To investigate into the effect of Puerarin on the activity of AGEs,HUVECs and eNOS and on MDA content.METHOD Isotope two-step chromatography was used to determine the activity of AGEs and eNOS and trace fluorometric method was used to determine MDA content.RESULT After AGEs stimulation,eNOS activity decreased while MDA markedly increased.In the Puerarin group(500 mg/L),eNOS activity resumed its normal level(P<0.05) and MDA level decreased correspondingly,but not significantly.CONCLUSION Puerarin can inhibit decrease of the activity of HUVECs-eNOS induced by AGEs,but it can not markedly reduce the content of MDA in the endothelial cell under the same condition.