葛根素对糖基化终产物干预eNOS活性及MDA生成的实验研究
Effect of Puerarin on Activity of AGEs, HUVECs and eNOS and on MDA Content
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摘要: 目的研究葛根素对糖基化终产物(AGEs)诱导的人脐静脉内皮细胞(HUVECs)内皮型一氧化氮合酶(eNOS)活性以及丙二醛(MDA)含量的影响。方法采用同位素二步色谱法测定AGEs刺激及葛根素作用后人脐静脉内皮细胞eNOS活性,用微量荧光法测定丙二醛(MDA)含量。结果AGEs刺激后eNOS活性降低,而细胞内过氧化产物丙二醛(MDA)显著增高,葛根素500 mg/L组干预后eNOS活性显著恢复(P<0.01),相应地,MDA含量降低,但并不具有显著性。结论葛根素能抑制AGEs诱导的HUVECs-eNOS活性下降,但并不能显著降低相同条件下内皮细胞内MDA含量。Abstract: OBJECTIVE To investigate into the effect of Puerarin on the activity of AGEs,HUVECs and eNOS and on MDA content.METHOD Isotope two-step chromatography was used to determine the activity of AGEs and eNOS and trace fluorometric method was used to determine MDA content.RESULT After AGEs stimulation,eNOS activity decreased while MDA markedly increased.In the Puerarin group(500 mg/L),eNOS activity resumed its normal level(P<0.05) and MDA level decreased correspondingly,but not significantly.CONCLUSION Puerarin can inhibit decrease of the activity of HUVECs-eNOS induced by AGEs,but it can not markedly reduce the content of MDA in the endothelial cell under the same condition.
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Keywords:
- AGEs /
- eNOS /
- Puerarin /
- endothelium /
- MDA
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