Box-Behnken设计-响应面法结合人工神经网络优化护肝解毒浸膏真空干燥工艺

Optimization of Vacuum Drying Process for Hugan Jiedu Extract Using Box-Behnken Design-Response Surface Methodology Combined with Artificial Neural Network

    摘要
  • 摘要:
    目的 优化护肝解毒浸膏的真空干燥工艺，确定最佳工艺参数。
    方法 采用HPLC法测定护肝解毒方中龙胆苦苷、绿原酸、芍药苷、盐酸小檗碱、五味子醇甲的含量。以这5种成分含量及干燥速率的总评归一化值（OD）作为综合评价指标。对浸膏相对密度、干燥厚度和干燥温度进行单因素考察，结合AHP-CRITIC 权重赋权法和 Box-Behnken 设计－响应面法（Box-Behnken design-response surface methodology，BBD-RSM）进行护肝解毒浸膏干燥工艺的优化试验。使用MATLAB R2024a深度学习工具箱构建人工神经网络（Artificial neural network，ANN），对 BBD-RSM 得出的浸膏相对密度、干燥厚度和干燥温度与OD值之间的关系进行预测和分析。
    结果 经BBD-RSM 结合ANN优化试验得出护肝解毒浸膏最佳干燥工艺参数为浸膏相对密度1.25、干燥厚度8 mm、干燥温度66 ℃，预测综合评分OD值为 0.821 1。在此条件下进行3次验证实验，测得龙胆苦苷、绿原酸、芍药苷、盐酸小檗碱、五味子醇甲的平均含量分别为5.97、4.75、5.89、1.83、0.47 mg·g-1，干燥平均速率为2.73 %·h-1。综合评分OD值分别为0.753 8、0.801 2、0.776 5，平均值为0.777 2，RSD为3.05%（<5%），与预测值接近。
    结论 优化的护肝解毒真空干燥工艺稳定可行，可为浸膏干燥成型工艺的质量控制提供参考。

     

    Abstract:
    OBJECTIVE To optimize the vacuum drying process of Hugan Jiedu extract and determine the optimal parameters.
    METHODS The contents of gentiopicroside, chlorogenic acid, paeoniflorin, berberine hydrochloride, and schisandrin A in the extract were determined by HPLC. The overall desirability (OD) value, derived from the five component contents and the drying rate, served as the comprehensive evaluation index. Following single-factor experiments on relative density, drying thickness, and temperature , the process was optimized using a hybrid AHP-CRITIC weighting method combined with Box-Behnken design-response surface methodology (BBD-RSM). An artificial neural network (ANN) was further employed to validate the predictive accuracy of the established model.
    RESULTS The optimal parameters were identified as relative density 1.25, drying thickness 8 mm and drying temperature 66 ℃, yielding a predicted OD value of 0.821 1. In three parallel verification trials under these conditions, the average contents of gentiopicroside, chlorogenic acid, paeoniflorin, berberine hydrochloride, and schisandrin were 5.97, 4.75, 5.89, 1.83, and 0.47 mg·g-1, respectively, with an average drying rate of 2.73%·h-1. The composite OD values were 0.7538, 0.8012, and 0.7765, respectively, yielding an average measured OD of 0.777 2 with an RSD of 3.05% (< 5%),which demonstrated high consistency with the predicted value.
    CONCLUSION The optimized vacuum drying process for Hugan Jiedu extract is stable and reproducible, providing a scientific basis for the standardized production and quality control of the extract.

     

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