前胡及其习用品的微性状与显微鉴别研究

Microscopic Identification of Micro-Traits and Microscopic Identification of Peucedani Radix and Its Common Varieties

  • 摘要:
    目的 系统研究前胡药材及其7种习用品的性状、微性状及显微特征,归纳其关键鉴别特征,为前胡药材及其习用品的有效鉴别提供参考依据。
    方法 运用性状、微性状及显微等鉴别方法,结合景深扩展成像及图像拼接等技术,得到前胡药材及其习用品的关键鉴别特征与高清图像,同时将部分特征进行数字化提取,并用SPSS26.0软件进行统计学分析。
    结果 得到前胡药材及习用品的高清彩色图像数据;其专属性鉴别特征为:性状鉴别项下的根头长度和环纹疏密、皮孔形态与面积、根的质地、折断面油点疏密等;显微鉴别项下的油室直径和数量、导管数量、有无韧皮纤维和木纤维等。统计学分析结果显示,前胡药材不同品种之间的皮孔面积、油室直径和密度、导管密度具有显著差异(P < 0.01)。
    结论 综合应用微性状和显微鉴别方法区分前胡药材及其习用品,特别是横切面偏振光全息彩色影像显微特征具有显著鉴别意义,同时将部分特征进行数字化提取并进行统计学分析,弥补了传统经验鉴别研究中受主观因素影响的不足,为前胡药材的流通、检验、临床用药、标准起草等工作提供参考。

     

    Abstract:
    OBJECTIVE To study the characteristics, micro-traits and microscopic characteristics of Peucedani radix and seven kinds of its common varieties and summarize the key identification characteristics so as to provide a reference for the effective identification of Peucedani radix and its common varieties.
    METHODS The key identification features and high-definition images of Peucedani radix and its common varieties were obtained by using the identification methods of traits, microtraits and microscopy, combined with the techniques of depth-of-field extended imaging and image stitching, and some of the features were digitally extracted and statistically analyzed by SPSS26.0 software.
    RESULTS The high-definition color image data of Peucedani radix and its common varieties were obtained. Its specific identification features were: root head length and annular sparseness, skin pore shape and area, root texture, and fracture surface oil spot density, etc. under the property identification; the diameter and number of oil chambers, the number of catheters, the presence or absence of bast fibers and wood fibers, etc. under the microscopic identification. The results of statistical analysis showed that there were significant differences in skin pore area, oil chamber diameter and density, and conduit density among different varieties of Peucedani radix (P < 0.01).
    CONCLUSION Micro-traits and microidentification methods can be comprehensively applied to distinguish Peucedani radix and its common varieties. In particular, the microscopic features of polarized light holographic color images in cross section have significant distinguishing significance, and some of the features are digitally extracted and statistically analyzed, which makes up for the shortcomings of subjective factors in the traditional empirical identification research, and provides a reference for the circulation, testing, clinical medication, and standard drafting of Peucedani radix.

     

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