Abstract: By systematically reviewing the development history of organoid technology and its application examples in Chinese medicine, this paper summarizes the specific applications of organoids in the mechanism and translation of Chinese medicine, pathological mechanisms, diagnostic techniques, and therapeutic strategies, and evaluate their advantages and limitations through literature analysis. Organoid technology provides an in vitro model that highly simulates the function of human organs for TCM research, and can simulate the multi-target and multi-pathway mechanism of action of TCM, which significantly improves the scientificity and precision of TCM in the analysis of disease mechanisms, drug screening and personalized treatment. However, it still faces challenges in standardization, ethical regulation, and clinical translation. The combination of organoid technology and TCM has a broad prospect, and it is necessary to further optimize the model construction, resolve ethical issues, and promote its wide application in TCM research and clinical practice through technological innovation, interdisciplinary cooperation, and international regulatory coordination in the future.