基于UPLC-Q-TOF-MS/MS技术的黑黄赤珠颗粒化学成分及入血成分分析

Analysis of Chemical Constituents and Blood-Absorbed Ingredients of Heihuang Chizhu Granules by UPLC-Q-TOF-MS/MS

    摘要
  • 摘要:
    目的 采用UPLC-Q-TOF-MS/MS技术对黑黄赤珠颗粒的化学成分及大鼠给药后的入血成分进行分析。
    方法 采用Waters ACQUITY UPLC HSS T3色谱柱(3 mm×100 mm, 1.8 μm)，以乙腈-0.1%甲酸为流动相梯度洗脱，在电喷雾离子源正、负离子模式下采集数据后与对照品保留时间、精确分子量、二级碎片离子以及参照相关文献进行化学成分鉴定。
    结果 从黑黄赤珠颗粒中共鉴定出104种化学成分，包括26个黄酮类、24个有机酸类、14个三萜类、8个萜类、7个苯丙素类、11个单帖苷类、14个其他类(酚类、生物碱类等)成分。在此基础上，从灌胃给药大鼠的血浆中鉴定出39个入血成分，包括28个原型，11个代谢产物。
    结论 首次分析鉴定了黑黄赤珠颗粒的化学成分及大鼠入血成分，为黑黄赤珠颗粒功效物质基础研究、过程质控标准建立等提供了科学依据。

     

    Abstract:
    OBJECTIVE To analyze the chemical constituents of Heihuang Chizhu Granules and the blood composition of rats after administration by UPLC-Q-TOF-MS/MS.
    METHODS A Waters ACQUITY UPLC HSS T3 column (3×100 mm, 1.8 μm) was eluted with acetonitrile-0.1% formic acid as mobile phase, and the data were collected in electrospray ion source positive and negative ion mode and then identified with the reference retention time, precise molecular weight, secondary fragment ions, and references to relevant literature.
    RESULTS A total of 104 chemical components were identified from Heihuang Chizhu Granules, including 26 flavonoids, 24 organic acids, 14 triterpenoids, 8 terpenoids, 7 phenylpropanoids, 11 monoglycosides, and 14 other components (phenols, alkaloids, etc.). On this basis, 39 blood-entering components were identified in the plasma of rats administered via gavage, including 28 prototypes and 11 metabolites.
    CONCLUSION The chemical constituents of Heihuang Chizhu Granules and the components entering the blood of rats are analyzed and identified for the first time in this study, and the results provide a scientific basis for the basic research of Heihuang Chizhu Granules and the establishment of process quality control standards.

     

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