层次分析结合响应面法优化煨大黄工艺及成分与色度相关性分析

AHP Combined with Response Surface Method to Optimize the Simmering Process of Rhei Radix et Rhizoma and Correlation Analysis between Composition and Color

  • 摘要:
    目的 研究大黄煨制工艺的最佳参数,探究煨大黄饮片粉末色度值与内在成分之间的相关性。
    方法 采用单因素-响应面法,对煨制温度、煨制时间、纸张用量与草木灰用量做单因素考察,在单因素试验基础上进行响应面试验,以外观性状及总蒽醌、游离蒽醌、浸出物、番泻苷A、番泻苷B含量为指标,采用层次分析法(AHP)对各项指标赋予权重,优选工艺。电子眼测定生品与煨品的色度值,用SPSS22.0软件进行相关性和回归分析,构建色度-成分回归方程。
    结果 大黄煨制最优工艺为140 ℃,5倍草木灰,2层湿纸包裹煨制2.5 h。
    结论 采用AHP结合响应面法优化的大黄煨制工艺合理可行,饮片粉末色度与成分含量具有显著相关性,构建的回归方程可靠,可通过色度预测饮片的内在成分含量。

     

    Abstract:
    OBJECTIVE To explore the optimal parameters of simmered Rhei Radix et Rhizoma and the correlation between the chroma values and the intrinsic composition of simmered Rhei Radix et Rhizoma decoction pieces powder.
    METHODS The single-factor-response surface method was used to investigate the simmering temperature, simmering time, paper dosage and plant ash dosage, the response surface experiment was carried out on the basis of the single factor experiment, the appearance traits, total anthraquinones, free anthraquinones, leachables, sennoside A and B contents were taken as indicators, the analytic hierarchy process (AHP) was used to give weights to each index, and the process was optimized. The chroma values of raw and simmered products were determined by electronic eye, the correlation and regression analysis were carried out by SPSS22.0 software, and the chroma-component regression equation was constructed.
    RESULTS The optimal process of simmering Rhei Radix et Rhizoma was 140 ℃, 5 times of plant ash, 2 layers of wet paper wrapped and being simmered for 2.5 h.
    CONCLUSION The simmering process of Rhei Radix et Rhizoma optimized by AHP combined with response surface method is reasonable and feasible, the color of decoction pieces has a significant correlation with the component content, and the regression equation constructed is reliable, which can predict the intrinsic component content of decoction pieces through chroma values.

     

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