基于UPLC-Q-TOF-MS鉴定玉屏风散入血成分

Identification of the Components of Yu-Ping-Feng-San Absorbed into Plasma Based on UPLC-Q-TOF-MS

    摘要
  • 摘要:
    目的 利用超高效液相色谱-四极杆-飞行时间串联质谱(UPLC-Q-TOF-MS)技术，分析玉屏风散乙醇提取物的入血成分，为玉屏风散的药效物质研究提供参考。
    方法 雄性BALB/c小鼠灌胃给予玉屏风散乙醇提取物(6.5 g·kg-1)，收集给药前(0 h)和给药后1、2、4、6、8、12 h的血浆样品，采用UPLC-Q-TOF-MS技术分析并鉴定吸收入血的成分。
    结果 共鉴定出玉屏风散乙醇提物中的73个化学成分，并在所有血浆样品中共鉴定出26个入血成分。入血成分中11个成分来自防风，15个成分来自黄芪。
    结论 较全面地分析玉屏风散乙醇提取物的化学成分和入血成分，为进一步阐明玉屏风散的药效物质基础及作用机制提供参考。

     

    Abstract:
    OBJECTIVE To analyze the plasma-entering components of the ethanol extract of Yu-Ping-Feng-San by ultra-performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UPLC-Q-TOF-MS) technology, so as to provide a reference for the study of the pharmacological substances of Yu-Ping-Feng-San.
    METHODS Male BALB/c mice were gavaged with the ethanol extract of Yu-Ping-Feng-San (6.5 g ·kg-1), and plasma samples were collected before administration (0 h) and 1, 2, 4, 6, 8, and 12 h after administration. The UPLC-Q-TOF-MS technology was used to analyze and identify the components absorbed into the plasma.
    RESULTS A total of 73 chemical components in the ethanol extract of Yu-Ping-Feng-San were identified, and 26 plasma-entering components were identified in all plasma samples. Among the plasma-entering components, 11 components were from Saposhnikoviae Radix, and 15 components were from Radix Astragali.
    CONCLUSION The chemical components and plasma-entering components of the ethanol extract of Yu-Ping-Feng-San were analyzed comprehensively, which provides a reference for further clarifying the pharmacological substance basis and mechanism of action of Yu-Ping-Feng-San.

     

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