基于UHPLC-Q Exactive Orbitrap-MS分析酸枣仁汤化学成分及雌性大鼠入血成分

Analysis of Chemical Components of Suanzaoren Decoction and Components Absorbed into Blood of Female Rats Based on UHPLC-Q Exactive Orbitrap-MS

    摘要
  • 摘要:
    目的 利用超高效液相色谱-串联质谱(UHPLC-Q Exactive Orbitrap-MS) 技术，分析酸枣仁汤灌胃给药后的入血成分。
    方法 以SD雌性大鼠为实验对象，灌胃给予酸枣仁汤，收集血清样品，采用UHPLC-Q Exactive Orbitrap-MS技术分析酸枣仁汤水提物和血清并鉴定吸收入血的原型成分和代谢产物, 并与LuMet-TCM数据库进行比对分析。
    结果 酸枣仁汤水提物共鉴定出458个成分，入血成分共鉴定出26个化学成分，其中23个为原型成分，3个为代谢产物。
    结论 研究发现的入血原型成分可能是酸枣仁汤的有效成分，为酸枣仁汤的药效物质基础研究提供参考。

     

    Abstract:
    Objective To analyze the blood components of Suanzaoren Decoction after oral administration using UHPLC-Q Exactive Orbitrap-MS technology.
    Methods Female Sprague-Dawley (SD) rats were used as experimental subjects, and Suanzaoren Decoction was administered orally. Serum samples were collected, and the aqueous extract of Suanzaoren Decoction and the serum were analyzed using UHPLC-Q Exactive Orbitrap-MS technology to identify the prototype components and metabolites absorbed into the blood by comparing and analyzing with the LuMet-TCM database.
    RESULTS It showed that a total of 458 components were identified in the aqueous extract of Suanzaoren Decoction, and 26 chemical components were identified in the blood, including 23 prototype components and 3 metabolites.
    CONCLUSION The prototype components absorbed into the blood discovered in this study may be the active ingredients of Suanzaoren Decoction, providing a reference for the research on the pharmacodynamic material basis of Suanzaoren Decoction.

     

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