功法静力性训练通过PINK1/Parkin通路调控T2DM小鼠骨骼肌线粒体自噬的作用机制

The Mechanism of Gongfa Static Training Regulating Mitophagy in Skeletal Muscle of Type 2 Diabetes Mellitus (T2DM) Mice via the PINK1/Parkin Pathway

    摘要
  • 摘要:
    目的 探讨功法静力性训练通过PTEN诱导的激酶1(PINK1)/帕金蛋白(Parkin)通路调控线粒体自噬在2型糖尿病(T2DM)骨骼肌胰岛素抵抗中的作用机制。
    方法 采用高脂饮食联合链脲佐菌素(STZ)腹腔注射建立T2DM小鼠模型，随机分为模型组、二甲双胍组、有氧运动组和功法静力性训练组。通过检测血糖、胰岛素抵抗指数(HOMA-IR)、糖化血红蛋白(HbA1c)、脂质代谢指标以及腓肠肌线粒体功能和PINK1、Parkin相关基因和蛋白表达，评估功法静力性训练的干预效果。
    结果 功法静力性训练显著降低了T2DM小鼠的FBG、糖化血红蛋白及胰岛素抵抗指数，改善了脂质代谢，并增强了胰岛素敏感性，通过上调PINK1、Parkin的mRNA和蛋白表达，改善腓肠肌线粒体的结构和功能。
    结论 功法静力性训练通过调控PINK1/Parkin通路，改善了T2DM小鼠骨骼肌线粒体功能和胰岛素抵抗。

     

    Abstract:
    OBJECTIVE To explore the mechanism by which Gongfa Static Training regulates mitophagy through the PTEN-induced kinase 1 (PINK1)/Parkin pathway in skeletal muscle insulin resistance in type 2 diabetes mellitus (T2DM).
    METHODS T2DM mouse model was established using a high-fat diet combined with streptozotocin (STZ) intraperitoneal injection. Mice were randomly divided into a model group, a metformin group, an aerobic exercise group, and a Gongfa Static Training group. The intervention effects of Gongfa Static Training were evaluated by measuring fasting blood glucose, Homeostatic Model Assessment of Insulin Resistance (HOMA-IR), glycated hemoglobin (HbA1c), lipid metabolism indicators, mitochondrial function in the gastrocnemius muscle, and the expression of PINK1 and Parkin-related genes and proteins.
    RESULTS Gongfa Static Training significantly reduced fasting blood glucose, HbA1c, and insulin resistance index in T2DM mice, improved lipid metabolism, and enhanced insulin sensitivity. It improved the structure and function of mitochondria in the gastrocnemius muscle by upregulating the mRNA and protein expression of PINK1 and Parkin.
    CONCLUSION Gongfa Static Training improves mitochondrial function and insulin resistance in the skeletal muscle of T2DM mice by regulating the PINK1/Parkin pathway.

     

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