OBJECTIVE To explore the anti-tumor effect of Yiqi Yangyin Jiedu Recipe on Lewis lung cancer mice and its influence on immunomodulation.

METHODS Lewis lung cancer mouse model was constructed by subcutaneous injection of Lewis lung cancer cells under the armpit and divided into model group, low dose group, medium dose group and high dose group. The tumor volume, tumor inhibition rate and survival rate were calculated. The apoptosis and DNA damage of tumor cells were detected by HE staining, TUNEL assay and comet assay respectively. The expression levels of IFN-γ, IL-2 and TNF-α were detected by ELISA. CD8⁺, CD8⁺/CD4⁺, PD1⁺, IFN-γ⁺, CD28⁺cells were detected by immunofluorescence and flow cytometry. The protein levels of PD1, CD69 and CD28 were detected by Western blot.

RESULTS Yiqi Yangyin Jiedu Recipe has anti-tumor effects, prolonging survival, promoting apoptosis of tumor cells and aggravating DNA damage of tumor cells. Drug treatment promoted the expression of IFN-γ, IL-2 and TNF-α, increased the proportion of CD8⁺T, CD8⁺/CD4⁺T, IFN-γ⁺and CD28⁺cells, and inhibited the proportion of PD1⁺cells and protein expression, and the protein levels of CD69 and CD28 were significantly increased.

CONCLUSION Yiqi Yangyin Jiedu Recipe can inhibit the development of tumor, and its regulation of immune function is the potential mechanism of its anti-tumor effect.