Abstract:
OBJECTIVE To enrich the polymethoxyflavonoid-rich fraction from Citri Reticulatae Pericarpium using D101 macroporous resin, and further to analyse the chemical constituents using High-Performance Liquid Chromatography Quadrupole-Time-of-Flight Mass Spectrometry (HPLC-Q-TOF-MS).
METHODS Citri Reticulatae Pericarpium extract was adsorbed on D101 macroporous resin and then eluted with different concentrations of ethanol to enrich the polymethoxyflavonoid-rich fraction; the content of the major flavonoid components was determined by high-performance liquid chromatography (HPLC); and the components were further analysed by HPLC-Q-TOF-MS.
RESULTS The established HPLC method for the simultaneous determination of seven flavonoids in Citri Reticulatae Pericarpium extract was accurate and reliable. The contents of compounds such as nobiletin and tangeritin were significantly increased in the polymethoxyflavonoid-rich fraction. Using high resolution mass spectrometry (HRMS), 70 and 60 compounds were identified from the Citri Reticulatae Pericarpium extract and the polymethoxyflavonoid-rich fraction, respectively, with 53 polymethoxyflavonoids being detected in these two extracts.
CONCLUSION The results of this study provide an experimental basis for further identification of the pharmacological substance basis of the polymethoxyflavonoids in Citri Reticulatae Pericarpium and the establishment of quality control methods.
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