Abstract: Based on the view of the correspondence between nature and man in traditional Chinese medicine (TCM), TCM master Professor Xia Guicheng attaches importance to the transformation of yin and yang in the female reproductive rhythm and created the "menstrual cycle rhythm regulation method". Women's special physiological phenomena are manifested in the periodic rhythm. The biological clock is the internal mechanism for producing and regulating the physiological behavior and laws of organisms. The biological clock gene is widely expressed in ovarian granulosa cells, theca cells and corpus luteum cells, and is involved in the synthesis of steroid hormones, differentiation of follicular somatic cells, follicle development and ovulation and other physiological processes, playing an important role in female reproductive endocrine. Circadian rhythm disorders can lead to reproductive diseases such as menstrual disorders, ovarian dysfunction, and endocrine metabolic disorders. There are similarities between TCM time medicine and the biological clock rhythm system. Fully understanding the impact of the biological clock on female ovarian function, revealing its scientific connotation, and clarifying the regulatory method and mechanism will have a positive role in resisting reproductive disorders and optimizing fertility.