香薷挥发油对吴茱萸有效成分透皮吸收的影响

Study on the Effect of Moslae Herba Oil on the Percutaneous Absorption of Active Ingredient of Evodia Rutaecarpa in vitro

    摘要
  • 摘要:
      目的  考察香薷挥发油对吴茱萸药效成分的促渗作用以及其促透皮吸收的能力。
      方法  采用扩散池和大鼠离体皮肤进行体外透皮实验, 以吴茱萸中多种成分作为指标, HPLC测定计算各成分累积透过量。HE染色、免疫组化染色、ATR-FTIR观察皮肤及角质层的变化, GC-MS测定挥发油给药后皮内贮存挥发油成分。
      结果  香薷挥发油对吴茱萸中多种药效成分均具有促渗作用, 与阳性药氮酮相比, 香薷挥发油对金丝桃苷、吴茱萸碱、吴茱萸次碱的累积透过量更大。且香薷挥发油中倍半萜成分及含量占比最大的麝香草酚更易在皮肤中贮存, 可以扰乱角质层脂质结构, 促进药物的渗透。
      结论  为透皮吸收促进剂的选择提供参考。

     

    Abstract:
      OBJECTIVE  To investigate whether Moslae herba oil has penetration enhancing effect on the active components of Evodia rutaecarpa and its ability to promote transdermal absorption.
      METHODS  In vitro transdermal experiments were performed using the diffusion pool and the isolated skin of rats. Using various components of Evodia rutaecarpa as indicators, HPLC was determined to calculate the umulative penetration amount of each component. Changes in the skin and cuticle layers were observed by HE staining, immunohistochemical staining, and ATR-FTIR. GC-MS was used to measure Moslae herba oil components after administration.
      RESULTS  Moslae herba oil showed the penetration-enhancing effect on various components in Evodia rutaecarpa. Compared with the postive drug nitrogenone, the cumulative penetration amounts of hyperoside, evodiamine and rutaecarpine were greater. In addition, the sesquiterpene composition and thymol in the Moslae herba oil were more likely to be stored in the skin, which could disturb the lipid structure of the cuticle layer and promote the penetration of drugs.
      CONCLUSION  The results provide reference for the selection of transdermal absorption agent.

     

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