荆花蜜质量控制指标与药用价值研究

Study on Quality Control Indicators and Medicinal Value of Vitex Honey

    摘要
  • 摘要:
      目的  对荆花蜜质量控制指标和药用价值进行研究。
      方法  收集近4年广西都安地区定地饲养模式生产的荆花蜜样品, 研究其理化特征和对盐酸哌洛丁胺诱导的小鼠传输障碍便秘模型的影响。
      结果  经蜜蜂充分酿造的荆花蜜中花粉以荆条花花粉粒为主, 水分可稳定达到20%以下, 电导率低于0.8 mS·cm-1，pH 3.67~3.93，游离酸27.3~32.3 meq·kg-1、内酯15.6~21.5 meq·kg-1、总酸35.4~53.7 meq·kg-1、淀粉酶17.6~21.5 U、羟甲基糠醛(5-HMF)3.3~11.6 mg·kg-1, 总酚含量450~650 mg·kg-1, 总黄酮含量16.3~26.6 mg·kg-1, DPPH清除EC50 67.5~90.1 mg·mL-1。薄层色谱研究结果显示, 荆花蜜与其他10余种蜂蜜呈现不同的特征图谱, 但不同年份荆花蜜的图谱特征相似; 每日饮用不同浓度的蜂蜜水溶液, 盐酸哌洛丁胺诱导的传输障碍小鼠便秘模型的便秘症状得到明显改善和缓解。
      结论  为荆花蜜各级各类质量标准制定和其药用价值研究进行了有益探索, 以期为后续标准制定和产品开发提供依据。

     

    Abstract:
      OBJECTIVE  To evaluate the quality control indicators and medicinal value of Vitex Honey.
      METHODS  Vitex honey samples from Du'an area of Guangxi in the past four years were collected, and their physicochemical characteristics and effects on the loperamide hydrochloride-induced transmission disorder constipation mouse models were studied.
      RESULTS  The pollen grains of the Vitex Honey were mainly from Vitex flower, with a stable water content of less than 20%, conductivity below 0.8 mS·cm-1, pH between 3.67 and 3.93, free acid 27.3 to 32.3 meq·kg-1, lactones 15.6 to 21.5 meq·kg-1, total acid 35.4 to 53.7 meq·kg-1, amylase 17.6 to 21.5 U, hydroxymethylfurfural (5-HMF) 3.3 to 11.6 mg·kg-1, total phenolic content 450 to 650 mg·kg-1, and total flavonoid content 16.3 to 26.6 mg·kg-1, DPPH clearance EC50 ranges from 67.5 to 90.1 mg·mL-1. The results of thin layer identification research showed that Vitex honey exhibited different characteristic spectra compared to more than ten other types of honey, but Vitex honey exhibited relatively consistent spectral characteristics in different years; daily intaking of different concentrations of honey aqueous solution significantly improved and alleviated the constipation symptoms of loperamide hydrochloride-induced transmission disorder constipation in mice.
      CONCLUSION  This study provides a beneficial exploration for the formulation of various quality standards for Vitex honey at all levels and the study of its medicinal value, in order to provide a basis for subsequent standard formulation and product development.

     

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