不同品系及成熟度宁夏枸杞叶资源化学研究及抗氧化活性评价

Chemical Composition Analysis and Antioxidant Activity Evaluation of Lycium barbarum Leaves with Different Strains and Maturity

    摘要
  • 摘要:
      目的  分析不同品系及不同成熟度宁夏枸杞叶中多类型资源性化学成分及抗氧化活性差异, 为其资源化利用提供依据。
      方法  采用UPLC-TQ-MS技术对不同成熟度的果用和叶用2种宁夏枸杞(宁杞7号、宁杞菜1号)叶中的黄酮类、酚酸类和生物碱类等19种主要化学成分进行分析测定, 利用1, 1-二苯基-2-三硝基苯肼(DPPH)及2, 2'-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基清除能力、铁离子还原能力评价其抗氧化活性。
      结果  叶芽和幼叶中各成分含量显著高于嫩叶和成熟叶。果用宁夏枸杞叶中芦丁含量较高, 而叶用宁夏枸杞叶中槲皮素-3-O-芸香糖-7-O-葡萄糖苷和甜菜碱含量较高。所有样品均呈现良好的抗氧化性, 且随着成熟度增加抗氧化活性均呈现减弱趋势。宁杞菜1号幼叶的DPPH清除能力最高, 叶芽的ABTS自由基清除能力和铁离子还原能力较强, 而宁杞7号叶芽3者均较高。叶用宁夏枸杞叶的抗氧化活性较果用宁夏枸杞叶高。宁夏枸杞叶抗氧化活性与隐绿原酸、5-O-阿魏酰奎宁酸、槲皮素-3-O-芸香糖-7-O-葡萄糖苷、绿原酸、甜菜碱含量呈显著正相关。
      结论  不同品系不同成熟度宁夏枸杞叶所含资源性成分含量及抗氧化活性差异较大, 果用和叶用宁夏枸杞叶芽与幼叶各成分含量均较高且均具有较好的抗氧化活性, 具有较高的开发应用价值, 叶用宁夏枸杞叶开发应用价值高于果用宁夏枸杞叶。研究结果可为不同品系及不同成熟度宁夏枸杞叶的资源化利用提供参考。

     

    Abstract:
      OBJECTIVE  To analyze the differences in multi-type resource chemical components and antioxidant activity of Lycium barbarum leaves (LBLs) with different strains and different maturity in order to provide the data support for their resource utilization.
      METHODS  The content of 19 main chemical components including flavonoids, phenolic acids and alkaloids in the leaves of two L. barbarum cultivars for fruit and leaf (Ningqi No.7 and Ningqicai No.1) with different maturities were determined by UPLC-TQ-MS technology. Free radical scavenging abilities of DPPH and ABTS, and iron ion reducing ability were used to evaluate the antioxidant activity of LBLs.
      RESULTS  The contents of various components in LBLs with different strains and different maturity were significantly different. The contents of each component in leaf buds and tender leaves were significantly higher than those in young leaves and mature leaves. The LBLs for fruit use have higher rutin content, while the LBLs for leaf use have higher contents of quercetin-3-O-rutose-7-O-glucoside and betaine. All samples showed good antioxidant properties, and the antioxidant activity showed a weakening trend with increasing maturity. The tender leaves of Ningqicai No. 1 had the highest DPPH scavenging ability, and the leaf buds had the highest ABTS free radical scavenging ability and iron reduction ability, while all three were higher in the leaf buds of Ningqi No. 7. The antioxidant activity of LBLs for leaf use was higher than that of LBLs for fruit use. The antioxidant activity of LBLs was significantly positively correlated with the contents of cryptochlorogenic acid, 5-O-feruloylquinic acid, quercetin-3-O-rutino-7-O-glucoside, chlorogenic acid and betaine.
      CONCLUSION  The content of resourceful components and antioxidant activity of LBLs varies greatly among different strains and different maturity levels. Leaf buds and tender leaves of L. barbarum have higher content of each component and better antioxidant activity, which show higher development and application values. The development and application value of LBLs for leaf use is higher than that of LBLs for fruit use. The results can provide reference for the resource utilization of LBLs with different strains and different maturity levels.

     

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