基于数据挖掘和网络药理学探究中医药治疗卵巢储备功能下降组方规律及作用机制

Medication Rule and Molecular Mechanism of Chinese Medicine in the Treatment of Diminished Ovarian Reserve Based on Data Mining and Network Pharmacology

    摘要
  • 摘要:
      目的  基于数据挖掘技术和网络药理学方法探索中医药治疗卵巢储备功能下降(DOR)的用药规律, 总结疾病特点和用药思路, 揭示临床常用药物的物质基础和作用机制。
      方法  从中国知网(CNKI)中收集中医药治疗卵巢储备功能下降相关临床文献, 提取方药信息, 建立用药数据库, 对证型分布、用药规律进行分析。运用网络药理学方法阐明具有补肾滋阴养血功效的高频药物组合治疗肝肾阴虚型卵巢储备功能下降的关键靶点和分子机制。
      结果  数据库筛选得到64首方剂, 120味中药, 得到治疗卵巢储备功能下降常用的强关联性药物组合熟地、当归、白芍、菟丝子、枸杞子、淫羊藿。6味高频药物组合含有活性成分40个, 治疗肝肾阴虚型卵巢储备功能下降靶点共163个, 涉及PGR、ESR1、VEGFA等。关键信号通路主要包括癌症通路、细胞因子及其受体的相互作用、孕激素介导的卵母细胞成熟等。
      结论  初步解析了中医治疗DOR谨守“肾虚”本质, 用药以补肾滋阴为主, 兼以理气、化瘀、健脾、疏肝为辅的配伍规律。并在分子水平揭示了具有补肾滋阴养血功效的高频药物组合治疗肝肾阴虚型DOR的机制。为后续治疗DOR药物开发和临床应用提供理论基础。

     

    Abstract:
      OBJECTIVE  To explore the medication patterns of traditional Chinese medicine in treating diminished ovarian reserve (DOR), summarize the characteristics of the disease and medication ideas, and reveal the material basis and mechanism of action of commonly used medicines in clinical practice by data mining technology and network pharmacology approach.
      METHODS  Collect clinical literature related to the treatment of DOR from China National Knowledge Infrastructure (CNKI). Extract prescription information, establish a medication database, and analyze the distribution of syndrome types and medication patterns. Use network pharmacology methods to elucidate the key targets and molecular mechanisms of high-frequency medicine combinations with the effects of tonifying the kidney and nourishing Yin and blood in the treatment of DOR with liver and kidney Yin deficiency syndrome.
      RESULTS  64 prescriptions and 120 traditional Chinese medicines were screened from the database. The commonly used and strongly correlated medicines for DOR treatment were Cuscuta chinensis, Rehmanniae Radix Praeparata, Angelica, Radix Paeoniae Alba, Epimedium brevicornu Maxim, Fructus Lycii. The combination of six high-frequency medicines contains 40 active components and 163 targets for treating DOR with liver and kidney Yin deficiency syndrome, involving PGR, ESR1, VEGFA, etc. The key signaling pathways mainly include cancer pathways, interactions between cytokines and their receptors, progestogen-mediated oocyte maturation, etc.
      CONCLUSION  This paper preliminarily analyzed the compatibility law of traditional Chinese medicine in treating DOR, adhering to the essence of "kidney deficiency", with nourishing liver and kidney essence and blood as the main treatment principle, and supplemented by regulating qi, resolving blood stasis, strengthening the spleen, and soothing the liver. At the molecular level, the mechanism of high-frequency medicine combination treatment for DOR with liver and kidney Yin deficiency syndrome was revealed, which provided a theoretical basis for the development and clinical application of subsequent DOR medicine.

     

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