Abstract: The mutual interference of clear and turbid qi refers to the imbalance between the rise and fall of clear qi and turbid qi: the failure of clear qi to rise and turbid qi to fall, and the obstruction of the transfer of fine substances, which become a summary of the pathogenesis of endogenous turbid pathogens. Bile acids can regulate the body's glucose and lipid metabolism and energy metabolism by activating bile acid receptors, which is an important way for the intestinal tract to regulate the homeostasis of glucose and lipid metabolism. It is proposed that the receptor of intestinal bile acid is the biological basis of the mutual interference of clear and turbid qi theory; the mutual interference of clear and turbid qi, as well as the endogenous turbid pathogens, are the key pathogenesis of glucose and lipid metabolism diseases in traditional Chinese medicine; and ascending clear qi and lowering turbid qi is an important treatment method in traditional Chinese medicine for the prevention and treatment of glucose and lipid metabolism diseases. Under the guidance of the mutual interference of clear and turbid qi theory, regulating the intestinal bile acid receptors to treat glucose and lipid metabolism diseases is of great value in clarifying the modern biological basis of the "mutual interference of clear and turbid qi" theory, and provides new ideas for TCM to treat glycolipid metabolic diseases.