基于脂质组学探究健脾利湿方对NAFLD小鼠肝脏脂质的调控作用

Using Lipidomics Technology to Investigate the Regulatory Impact of Jianpi Lishi Formula on Lipid Metabolism in NAFLD Mice

    摘要
  • 摘要:
      目的  基于LC-MS脂质组学探讨健脾利湿方对非酒精性脂肪肝(NAFLD)小鼠肝脏脂质代谢的调控作用。
      方法  将C57BL/6J小鼠随机分为空白组、模型组、健脾利湿方组3组, 每组6只, 采用60%高脂饲料饲喂12周诱导NAFLD模型, 同时进行给药干预。通过生化指标检测及病理切片进行药效学评估, LC-MS脂质组学检测小鼠肝脏组织脂质变化。
      结果  健脾利湿方改善了HFD诱导的体质量增加, HE、油红染色显示具有肝脏保护作用, 可恢复葡萄糖代谢水平。其次, 降低了肝脏Fasn、CD36、SCD1、Srebp1c的mRNA水平(P < 0.05, P < 0.01，P < 0.001);此外, 脂质组学研究发现, 健脾利湿方对肝脏脂质组成存在调控作用。
      结论  健脾利湿方具有降低体质量、调节血糖、保护肝脏的作用, 脂质组学研究表明对肝脏脂质组成的调控可能是健脾利湿方治疗NAFLD主要作用机制。

     

    Abstract:
      OBJECTIVE  To explore the regulatory effect of Jianpi Lishi Formula on liver lipid metabolism in NAFLD mice based on LC-MS lipidomics.
      METHODS  C57BL/6J mice were randomly divided into three groups: blank group, model group, and Jianpi Lishi Formula group, with 6 mice in each group. NAFLD models were induced by feeding them 60% high-fat feed for 12 weeks, during which medication intervention was also performed. The pharmacodynamic evaluation was conducted through biochemical indicator detection and pathological sections, while LC-MS lipidomics was used to detect lipid changes in mouse liver tissue.
      RESULTS  Jianpi Lishi Formula improved the increase in body mass induced by HFD. HE and oil red staining showed that it had a liver protective effect and could restore glucose metabolism levels. Also, Jianpi Lishi Formula decreased the mRNA levels of Fasn, CD36, SCD1, and Srebp1c in the liver (P < 0.05, P < 0. 01, P < 0. 001). Lipidomics research found that Jianpi Lishi Formula has a regulatory effect on liver lipid composition.
      CONCLUSION  Jianpi Lishi Formula has the effects of reducing body mass, regulating blood sugar, and protecting the liver. Lipidomics study indicates that the relief of NAFLD induced by Jianpi Lishi Formula may be related to the regulation of liver lipid composition.

     

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