OBJECTIVE  Based on the characteristic spectrum combined with the determination of effective Toxiciy appearance character, to study the changes of toxic components in the processing of Aristolochia contorta Bge.

  METHODS  The characteristic chromatogram of 17 batches of Aristolochia contorta Bge. was established by HPLC, and the parameters of honey, dilution times, humid time and frying time were investigated. Samples were taken at intervals in the process, the chromaticity value and characteristic map were measured, and the comprehensive evaluation of appearance characters and intrinsic toxicity values was carried out.

  RESULTS  10 common peaks were calibrated and 6 characteristic peaks were identified in the characteristic spectrum of Aristolochia contorta Bge., among which peak 2 was magnoflorine, peak 5 was 7-hydroxy aristolochic acid A, peak 6 was aristolochic acid D, peak 8 was aristolochic acid B, peak 9 was aristolochic acidⅠ, and peak 10 was aristolochic acidⅠ. According to the pre-treatment investigation, the dosage of honey was 25 kg of refined honey per 100 kg of products to be roasted, the honey was diluted by 3 times amount of water, and the humid time was 60 min. The processing process showed that the peak area of the toxic components of Aristolochia contorta Bge. decreased obviously with the processing time, and the toxic value of magnoflorine and five toxic components gradually increased. According to the comprehensive evaluation of appearance traits and intrinsic toxicity value, the samples processed for 19-27 min ranked in the top five, processing time was determined to be 23-27 min.

  CONCLUSION  After Aristolochia contorta Bge. is processed, the relative contents of toxic components decreases, while the toxic value increases. The quality evaluation method of effective toxicity value combined with appearance characteristics can provide reference for the processing of toxic Chinese herbal pieces