吉春亮, 苏艳, 张金戈, 盛先森, 张健榕, 单晨啸. 基于UPLC-Q-TOF-MS/MS技术分析前胡化学物质组及体内成分[J]. 南京中医药大学学报, 2022, 38(12): 1143-1155. DOI: 10.14148/j.issn.1672-0482.2022.1143
引用本文: 吉春亮, 苏艳, 张金戈, 盛先森, 张健榕, 单晨啸. 基于UPLC-Q-TOF-MS/MS技术分析前胡化学物质组及体内成分[J]. 南京中医药大学学报, 2022, 38(12): 1143-1155. DOI: 10.14148/j.issn.1672-0482.2022.1143
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JI Chunliang, SU Yan, ZHANG Jinge, SHENG Xiansen, ZHANG Jianrong, SHAN Chenxiao. Identification of the Chemical Components of Peucedani Radix and in vivo Chemicals Based on UPLC-Q-TOF-MS/MS[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(12): 1143-1155. DOI: 10.14148/j.issn.1672-0482.2022.1143
Citation: JI Chunliang, SU Yan, ZHANG Jinge, SHENG Xiansen, ZHANG Jianrong, SHAN Chenxiao. Identification of the Chemical Components of Peucedani Radix and in vivo Chemicals Based on UPLC-Q-TOF-MS/MS[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(12): 1143-1155. DOI: 10.14148/j.issn.1672-0482.2022.1143
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基于UPLC-Q-TOF-MS/MS技术分析前胡化学物质组及体内成分

Identification of the Chemical Components of Peucedani Radix and in vivo Chemicals Based on UPLC-Q-TOF-MS/MS

    摘要
  • 摘要:
      目的  通过UPLC-Q-TOF-MS/MS研究前胡的化学物质组, 探究其被吸收入血和肺组织中的原型成分及代谢物。
      方法  采用Thermo AcclaimTM RSLC 120 C18柱(3.0 mm×100 mm, 2.2 μm), 柱温: 35 ℃, 以0.1%甲酸水-乙腈为流动相进行梯度洗脱, 流速为0.3 mL · min-1, 进样量为5 μL。采用电喷雾(ESI)离子源, 正离子模式下采集质谱信息。
      结果  根据化合物的精确m/z、二级碎片等质谱信息, 结合标准品的质谱信息和比对文献检索信息, 最终在前胡提取液中共推测出54个化学成分, 包括52个香豆素类化合物、1个萜烯类化合物和1个酚类化合物; 给药后在大鼠血浆中共鉴定出21个原型成分和26个代谢物, 肺组织中共鉴定出17个原型成分和9个代谢物。
      结论  该方法快速、准确、全面, 其分析结果为前胡的质量控制以及进一步的药效物质基础研究奠定了基础。

     

    Abstract:
      OBJECTIVE   To deduce the main chemical components in Peucedani radix and its absorbed prototype components and metabolites in plasma and lung using a UPLC-Q-TOF-MS/MS method.
      METHODS   Thermo AcclaimTM RSLC 120 C18 column (3.0 mm×100 mm, the particle size is 2.2 μm) was used by using a gradient elution program with 0.1% formic acid water and acetonitrile as mobile phase. Column temperature was set at 35 ℃, the flow rate was 0.3 mL · min-1 and the injection volume was 5 μL. ElectroSpray Ionization Source (ESI) was selected to collect parent and product mass spectrometric data in positive ion mode.
      RESULTS   The chemical components in Peucedani radix were analyzed and identified according to the accurate mass, retention time, the fragmentation and other information of the compounds, especially, by comparing the reference substances and literature data. A total of 54 chemical constituents were identified in Peucedani radix, including 52 coumarins, 1 terpene and 1 phenolic compound. And a total of 21 prototype components and 26 metabolites were identified in rat plasma. 17 prototype components and 9 metabolites were identified in lung tissue after oral administration of Peucedani radix extract to rats.
      CONCLUSION   This method is rapid, accurate and comprehensive. The analysis results lay a foundation for the quality control of Peucedani radix and the further study of pharmacological material basis.

     

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