胆南星拮抗热性惊厥模型小鼠脑组织损伤及炎症的作用研究

曾平; 闫玉梅; 郁红礼; 吴皓; 陶兴宝; 谢雨薇; 程砚秋; 王彩霞; 王贺鹏; 陈劲松

doi:10.14148/j.issn.1672-0482.2022.0520

胆南星拮抗热性惊厥模型小鼠脑组织损伤及炎症的作用研究

Effect of Arisaema Cum Bile on Brain Tissue Injury and Inflammation in Febrile Seizures Model Mice

摘要

摘要:
目的研究胆南星拮抗热性惊厥模型小鼠脑组织损伤及炎症的药效作用。
方法采用干酵母混悬液及戊四氮溶液构建热性惊厥小鼠模型, 考察不同剂量胆南星水提物对模型小鼠肛温、惊厥潜伏期及惊厥持续时间的影响, HE染色观察小鼠海马神经元形态, ELISA法检测小鼠血清cAMP、PGE₂水平, Western blot法检测脑组织COX-2、iNOS、γ-氨基丁酸A型受体(GABAAR)、胶质纤维酸性蛋白(GFAP)蛋白表达, qPCR法检测海马组织炎症因子IL-1β、IL-6、TNF-α mRNA表达。
结果与模型组比较, 胆南星高剂量组能够降低模型小鼠肛温(P < 0.05), 延长惊厥潜伏期(P < 0.05), 缩短惊厥持续时间(P < 0.01), 上调GABAAR蛋白表达(P < 0.05);同时降低血清cAMP、PGE₂水平(P < 0.01), 改善海马神经元细胞形态学变化, 下调脑组织COX-2、iNOS、GFAP蛋白表达(P < 0.05), 降低海马组织炎症因子IL-1β、IL-6、TNF-α mRNA表达水平(P < 0.05)。
结论胆南星具有良好的抗热性惊厥作用, 其机制可能与解热、拮抗脑组织炎症有关。

Abstract:
OBJECTIVE To study the efficacy of Arisaema Cum Bile on antagonizing brain tissue damage and inflammation in febrile seizures model mice.
METHODS Mice model of febrile seizures was established by using dry yeast suspension and pentylenetetrazol solution, and the effects of low, medium and high dose groups of Arisaema Cum Bile water extract on rectal temperature, convulsion latency and convulsion duration of model mice were investigated. HE staining was used to observe the morphology of mice hippocampal neurons. ELISA was used to detect the levels of serum cAMP and PGE₂. Western blot method was used to detect the protein expressions of COX-2, iNOS, GFAP and GABAAR in brain tissue. The qPCR method was used to detect inflammatory factors IL-1β, IL-6, TNF-α mRNA expression in hippocampus tissue.
RESULTS Compared with the model group, the high-dose Arisaema Cum Bile group could reduce the rectal temperature (P < 0.05), prolong the convulsion latency (P < 0.05), shorten the duration of convulsions (P < 0.01), and up-regulate the expression of GABAAR protein (P < 0.05); At the same time, it decreased the levels of serum cAMP and PGE₂ (P < 0.01), and improved the morphological changes of hippocampal neurons, down-regulated the expressions of COX-2, iNOS and GFAP protein in the brain tissue (P < 0.05), and decreased the expression levels of IL-1β, IL-6 and TNF-α mRNA in the hippocampus (P < 0.05).
CONCLUSION Arisaema Cum Bile has a good antipyretic convulsant effect, and its mechanism may be related to antipyretic and antagonizing brain tissue inflammation.

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