二至丸对绝经后骨质疏松模型大鼠脂肪组织糖代谢影响

The Effect of Erzhiwan on Glucose Metabolism of Adipose Tissue in Postmenopausal Osteoporosis Model Rats

    摘要
  • 摘要:
      目的  探讨二至丸对绝经后骨质疏松症大鼠脂肪组织糖代谢的影响。
      方法  通过切除双侧卵巢建立绝经后骨质疏松症大鼠模型, 大鼠随机分为: 模型组、阿仑膦酸钠组、氟化钠组和二至丸组, 每组8只。另设假手术组8只。分别予生理盐水(5 mL·kg-1)，阿仑膦酸钠(1 mg·kg-1)，氟化钠(5 mg·kg-1)，二至丸(1.6 g·kg-1)灌胃8周, 每日1次, 假手术组干预同模型组。采用ELISA法检测血清胰岛素，qPCR、Western blot法检测脂肪组织Glut4、PGC-1α、Sirt1 mRNA及蛋白表达。
      结果  与假手术组相比, 模型组大鼠血清胰岛素明显增加(P < 0.05), 脂肪组织Glut4、PGC-1α及Sirt1 mRNA和蛋白表达水平显著减少(P < 0.01)。与模型组相比, 二至丸组血清胰岛素明显减少, 脂肪组织Glut4、PGC-1α及Sirt1 mRNA及蛋白表达水平显著增加(P < 0.01)。
      结论  二至丸能够激活Sirt1/PGC-1α/Glut4通路，改善绝经后骨质疏松症状态下脂肪组织糖代谢紊乱。

     

    Abstract:
      OBJECTIVE  To explore the effect of Erzhiwan on glucose metabolism of adipose tissue in postmenopausal osteoporosis model rats.
      METHODS  The postmenopausal osteoporosis rat model was established by bilateral ovariectomy. All model rats were randomly divided into 4 groups: model group, alendronate sodium group, sodium fluoride group and Erzhiwan group, with 8 rats in each group. Another 8 rats were set in sham operation group. Each group was given normal saline (5 mL·kg-1), alendronate sodium (1 mg·kg-1), sodium fluoride(5 mg·kg-1) and Erzhiwan (1.6 g·kg-1) once a day for 8 weeks, respectively. The sham group was given the same intervention as the model group. Serum insulin was detected by ELISA. The expressions of Glut4, PGC-1α, Sirt1 mRNA and protein in adipose tissue were detected by qPCR and Western blot.
      RESULTS  Compared with the sham group, the serum insulin in the model group increased significantly, and the mRNA and protein expressions of Glut4, PGC-1α and Sirt1 in adipose tissue decreased significantly. Compared with the model group, the serum insulin in Erzhiwan group decreased significantly, and the mRNA and protein expressions of Glut4, PGC-1α and Sirt1 in adipose tissue increased significantly.
      CONCLUSION  Erzhiwan can activate Sirt1/PGC-1α/Glut4 to improve the disorder of glucose metabolism in adipose tissue under postmenopausal osteoporosis.

     

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