OBJECTIVE  To optimize the preparation method of hawthorn leaf extract phospholipid complex and research its physicochemical properties.

  METHODS  The mass fraction weight coefficient method was used to characterize the overall properties of the flavonoids of the hawthorn leaf extract. On the basis of single factor investigation, the orthogonal design experiment was used to optimize the preparation method, taking the component compound rate and mass compound rate as the index. It was characterized by scanning electron microscopy, UV spectrum, infrared spectrum and X-ray diffraction. While its apparent oil-water partition coefficient was also investigated.

  RESULTS  The best preparation method of hawthorn leaf extract phospholipid complex is to use absolute ethanol as the compound solvent, the mass concentration of hawthorn leaf extract is 1 mg ·mL^-1, the drug-to-lipid ratio is 1 ∶ 4, and the reaction is compounded at 60 ℃ for 1.5 h. Scanning electron microscopy, X-ray diffraction analysis, etc. verified the formation of hawthorn leaf extract phospholipid complex, and the apparent oil-water partition coefficient of different pH was improved compared with the crude drug.

  CONCLUSION  The preparation method of hawthorn leaf extract phospholipid complex is reasonable and feasible, and the fat solubility is improved.