潜阳育阴颗粒对自发性高血压大鼠肾脏足细胞损伤的影响及其机制研究

Mechanism of Qianyang Yuyin Granule on Renal Podocytes Injury in Spontaneously Hypertensive Rats

    摘要
  • 摘要:
      目的  探讨潜阳育阴颗粒(QYYY)对高血压肾损害的治疗作用及其作用机制。
      方法  以自发性高血压大鼠(SHR)为动物模型，潜阳育阴颗粒(低、中、高剂量)为干预，HE染色及Masson染色观察各组大鼠肾脏病理情况，Western Blot检测TRPC6及足细胞损伤相关蛋白Desmin、Nephrin和Podocin的表达。细胞实验用潜阳育阴含药血清低、中、高剂量(QYYYL、QYYYM、QYYYH)干预血管紧张素Ⅱ(Ang Ⅱ)诱导的大鼠足细胞损伤模型，Western Blot检测TRPC6及足细胞损伤相关蛋白的表达。免疫荧光观察TRPC6、Desmin的表达。
      结果  HE染色及Masson染色观察到潜阳育阴颗粒能明显改善SHR的肾损害，与模型组相比，QYYY能明显降低TRPC6、Desmin，升高Nephrin、Podocin表达水平及改善Ang Ⅱ所诱导的足细胞损伤(P < 0.05, P < 0.01)。
      结论  QYYY可以改善高血压肾损害，其机制可能与TRPC6及足细胞损伤相关蛋白的表达有关。

     

    Abstract:
      OBJECTIVE  To investigate the therapeutic effect of Qianyang Yuyin Granule (QYYY) on hypertensive kidney injury.
      METHODS  spontaneously hypertensive rats (SHR) were chosen as the animal model to evaluate the QYYY therapeutic effect. HE and Masson staining were used to observe the kidney pathology in each treatment group. The expression of TRPC6 and podocyte damage-related proteins Desmin, Nephrin and Podocin was determined by Western blot assay. In addition, low, medium and high doses (QYYYL, QYYYM, QYYYH) of Qianyang Yuyin drug-containing serum were used to treat an in vitro rat podocyte injury model induced by angiotensin Ⅱ (Ang Ⅱ). Similarly, Western blot and immunofluorescence assays were used to determine the expression of TRPC6 and podocyte damage-related proteins.
      RESULTS  HE and Masson staining indicated that QYYY treatment markedly alleviated the kidney damage in SHR, which correlates with significant reduction of TRPC6 and Desmin, and induction of Nephrin and Podocin, as well as the improvement of Ang Ⅱ-induced podocyte injury.
      CONCLUSION  QYYY can ameliorate kidney and podocyte damage, and reduce hypertensive kidney damage.

     

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