Abstract:
OBJECTIVE To observe the effect of Bushen Jianpi formulation on ER/c-Myc/TERT signaling pathway in mice with premature ovarian failure.
METHODS Fifty B6AF1 mice were randomly divided into normal group, model group, positive group, Bushen Jianpi formulation high-dose group and Bushen Jianpi formulation low-dose group. ZP3 was used to induce the establishment of premature ovarian failure model. Each group was given drug or normal saline, respectively, and the changes of oestrous cycle were observed regularly during the period. After 4 weeks of intervention, serum and ovarian tissue were collected. The levels of serum E2 and FSH were detected by radioimmunoassay. The pathological morphology of ovarian tissue was observed by HE staining. The changes of zona pellucida of ovary were detected by immunofluorescence assay. The expressions of ER, c-Myc and TERT were detected by Western blot.
RESULTS Compared with model group, Bushen Jianpi formulation high-dose and low-dose groups could significantly increase serum E2 level, decrease FSH level, significantly increase the number of growing follicles and mature follicles, significantly reduce the fluorescence expression of ovarian zona pellucidum, and significantly increase the expressions of ER, c-Myc and TERT.
CONCLUSION Bushen Jianpi formulation can improve the level of estrogen, promote the secretion of ER, stimulate the expression of c-Myc, regulate the expression of TERT, so as to promote the proliferation and development of ovarian granulocytes and the maturation of oocytes, which is the way to play its role in the treatment of premature ovarian failure.