Abstract: OBJECTIVE  To study the effect of Pudilan Xiaoyan Oral Liquid on energy metabolism in mice with lipopolysaccharide(LPS)-induced acute pneumonia.METHODS  ICR mice were randomly divided into control group, model group, Pudilan group and positive drug group, and the drugs were given orally for 5 days. GC-MS was used to detect 12 metabolites(pyruvic acid, lactic acid, succinic acid, fumaric acid, malic acid, α-ketoglutarate, phosphoenolpyruvic acid, cis-aconitic acid, 3-phosphoglyceric acid, citric acid, isocitric acid, glucose) in glycolysis and tricarboxylic acid(TCA) cycle in mouse serum and then multivariate data analysis was carried out to search the significant metabolites.RESULTS  Among the targeted metabolites, lactic acid, succinic acid, malic acid, α-ketoglutarate, isocitrate and glucose were found as the significant metabolites. Compared to the model group, these metabolites were effectively restored by Pudilan Xiaoyan Oral Liquid.CONCLUSION  This study accurately finds six differential metabolites related to inflammation, providing a reference for in-depth explanation of the mechanism of anti-respiratory inflammation of Pudilan Xiaoyan Oral Liquid at the level of energy metabolism.