葡萄籽原花青素对变形链球菌的体外抗菌活性研究
Antibacterial Activities of Grape Seed Procyanidins Against Streptococcus Mutans in Vitro
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摘要: 目的 研究葡萄籽原花青素（Grape seed procyanidins，GSPC）对变形链球菌的体外抗菌活性，探讨其防龋潜能。方法 采用微量肉汤法测定最低抑菌浓度（MIC）、最低杀菌浓度（MBC）、最低抑制生物膜浓度（MBIC）；采用结晶紫半定量法测定生物膜生成量；硫酸-蒽酮法测定产糖量，pH计测定产酸量。结果 葡萄籽原花青素的MIC值12.5 mg/mL；MBC值和MBIC值均为50 mg/mL；GSPC（≥12.5 mg/mL）降低变形链球菌生物膜的生成量，8倍MIC浓度（100 mg/mL）时抑制率达76%；GSPC在低于MIC浓度时仍具有抑制变形链球菌产糖、产酸能力。结论 葡萄籽原花青素具有对变形链球菌的体外抗菌活性，并能抑制变形链球菌的生物膜和致龋毒力因子。Abstract: OBJECTIVE To study the antibacterial activity of grape seed procyanidins (GSPC) against streptococcus mutans in vitro and to explore its anti-caries potential. METHODS Broth micro-dilution method was used to determine minimal inhibitory concentration (MIC)， minimal bactericidal concentration (MBC) and minimal biofilm inhibitory concentration (MBIC); crystal violet semi-quantitative method was used to determine biofilm-formation; anthrone sulfuric acid method was used to test glucan production and pH was used to test acid generation. RESULTS MIC value of GSPC was 12.5 mg/mL while MBC value and MBIC value were both 50 mg/mL. GSPC (≥12.5 mg/mL) could reduce biofilm formation， and especially when its content increased to eight times of MIC content(100 mg/mL)， the inhibition rate was able to achieve 76%. Also it had the ability of inhibiting the glucan production and acid generation at lower content than that of MIC. CONCLUSION GSPC shows antibacterial activity and inhibitory effects on biofilm-formation and cariogenic virulence factors against streptococcus mutans in vitro.
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Key words:
- grape seed procyanidins (GSPC) /
- streptococcus mutans /
- antibacterial activity /
- in vitro
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