痛泻要方对溃疡性结肠炎大鼠结肠黏膜ERK1、ERK2基因表达的影响

竺平; 谷云飞; 杨柏霖; 林秋; 陈红锦; 孙桂东; 丁义江

痛泻要方对溃疡性结肠炎大鼠结肠黏膜ERK1、ERK2基因表达的影响

1.
南京中医药大学第一临床医学院，江苏南京 210023
2.
南京中医药大学附属医院，江苏南京 210029
3.
南京中医药大学第三附属医院，江苏南京 210001

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出版历程
- 收稿日期: 2013-05-11
- 修回日期: 2013-06-15
- 刊出日期: 2013-07-10

The Inhibitory Effect of Toxic Chinese Drugs on the Growth of Human Colon Cancer Cell Line HCT116 and SW480

1.
The First Clinical College, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China
3.
The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210001, China

摘要

摘要: 目的从已知具有抗肿瘤作用的有毒中药或中药化合物中筛选出具有抑制不同发生途径人结肠癌细胞作用的药物。方法应用MTT法检测白花蛇舌草、雷公藤、重楼、龙葵、藤黄、天南星、黄药子、华蟾素、斑蝥酸钠及亚砷酸氯化钠对不同发生途径的人结肠癌细胞HCT116（hMLH1缺失结肠癌细胞株）和SW480（错配修复基因正常的结肠癌细胞株）生长抑制作用。结果斑蝥酸钠、华蟾素、亚砷酸氯化钠及藤黄提取物对人结肠癌细胞HCT116及SW480的生长有抑制作用。斑蝥酸钠、华蟾素及藤黄提取物干预HCT116细胞的IC₅₀值和干预SW480细胞的IC₅₀值比较有统计学差异（P<0.05），亚砷酸氯化钠干预两细胞株的IC₅₀值无统计学差异（P>0.05）。白花蛇舌草、雷公藤、重楼、龙葵、天南星、黄药子这6种中药的提取物在本实验条件下无效。结论藤黄提取物、斑蝥酸钠、亚砷酸氯化钠及华蟾素可有效抑制不同途径的人结肠癌细胞的生长，其中斑蝥酸钠、华蟾素、藤黄提取物可能具有特异性抗错配修复基因缺失所致结、直肠癌的作用。
- 有毒中药 /
- 人结肠癌 /
- HCT116细胞 /
- SW480细胞 /
- MTT
Abstract: OBJECTIVE To screen out the medicines that can be specifically against human colon cancer cells from the already known toxic antineoplastic Chinese drugs.METHODS Oldenlandia diffusa， Tripterygium wilfordii， Paris polyphylla， Solanum nigrum， Gamboges， Rhizoma arisaematis， Dioscorea bulbifera L， Cinobufacini， Sodium cantharidinate and Sodium arsenic were applied to treat the human colon cancer cell line HCT116(MLH1-deficient) and SW480(proficient mismatch repair)， MTT were used to analyze the growth inhibition of two cell lines.RESULTS Sodium cantharidinate， Cinobufacini， Sodium arsenic and Gamboges can inhibit cell growth of HCT116 and SW480， The IC50 values of HCT116 cell line were significantly lower than SW480 cell line when they were both treated with Sodium cantharidinate Cinobufacini and Gamboges(P<0.05). Meanwhile， there were no significant difference between the IC50 values of HCT116 cell line and SW480 cell line when they were treated with Sodium arsenic(P>0.05). However， Oldenlandia diffusa， Tripterygium wilfordii， Paris polyphylla， Solanum nigrum， Rhizoma arisaematis， Dioscorea bulbifera L did not present growth inhibition effect on HCT116 and SW480 in this study.CONCLUSION Gamboges， Sodium cantharidinate， Sodium arsenic and Cinobufacini can effectively inhibit the growth of human colon cancer cells developing from different genetic pathways. Gamboges， Sodium cantharidinate and Cinobufacini may specifically against MMR-deficient human colon cancer cells.
- toxic Chinese medicines /
- human colon cancer /
- HCT116 /
- SW480 /
- MTT

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参考文献(8)

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