Mechanisms of Combination ofAstragali Radix andSpatholobi Caulis in Treatment of Leukopenia Based on Network Pharmacology

OBJECTIVE To investigate the molecular mechanism ofAstragali Radix andSpatholobi Caulis in the treatment of leukopenia by network pharmacology approaches. METHODS The chemical active components ofAstragali Radix andSpatholobi Caulis were collected by Traditional Chinese Medicine Systems Pharmacology （TCMSP） Database， while the target genes for leukopenia and active components were collected by Genecards and CTD databases. The drug targets were mapped to disease targets， the protein-protein interaction network (PPI) of compound-disease was established. The hub genes of the PPI network were screened out. The compound-disease targets network model was visualized by Cytoscape software. Gene Ontology (GO) enrichment and KEGG signaling pathway analysis were generated by DAVID database. RESULTS 19 active components ofAstragali Radix andSpatholobi Caulis， and 108 common targets in treatment of leukopenia were obtained. The core targets were PIK3CA， IL-6， TNF， IL-2， IL-10. KEGG pathway enrichment showed multiple signaling pathways were involved， such as drug metabolism， cytokine-cytokine receptor interaction，hematopoietic cell lineage， JAK-STAT signaling pathway and so on. CONCLUSION The combination application ofAstragali Radix andSpatholobi Caulis in the treatment of leukopenia act on several key targets， such as PIK3CA， IL-6， TNF， IL-2， IL-10， to regulate cytokine-cytokine receptor interaction，drug metabolism， hematopoietic cell lineage and JAK-STAT signaling pathway.