Study on the Therapeutic Effect of Liuling Jiedu Wan on Acute Pharyngitis

OBJECTIVE To study the therapeutic effect of Liuling Jiedu Wan on acute pharyngitis. METHODS The model of acute pharyngitis was prepared by spraying 15% ammonia water into the young rats' pharynx. Rats with successful modeling were randomly divided into 5 groups， namely model group， Lanqin Oral Liquid group and Liuling Jiedu Wan group with 3 doses. Rats in the control and model groups were given normal saline by gavage. The drugs were given once a day by gavage continuously for 7 days. During the course of gavage， body weight， laryngeal redness and other signs of the rats were observed. After administration， HE staining was used to observe the pharyngeal lesions in rats， and the expression of inflammatory factors in the serum and the connective tissue under the pharyngeal mucosa were detected. The anti-inflammatory effect of Liuling Jiedu Wan was studied by capillary permeability and ear swelling in mice. RESULTS Compared with the control group， rats in the model group frequently scratched the oropharynx and experienced slow weight gain， obvious pharyngeal redness and swelling. Their tissue structures were abnormal， with a large number of connective tissue hyperplasia and inflammatory cell infiltration. After the administration of Liuling Jiedu Wan， the weights of the rats increased rapidly， the degrees of pharyngeal redness and swelling decreased， and the tissue structures were significantly improved. The structures of submucosal glands were normal， and no connective tissue hyperplasia and inflammatory cell infiltration were observed. Moreover， Liuling Jiedu Wan could significantly decrease the levels of IL-1β， IL-6 and TNF-α in serum and tissues of rats (P<0.05，P<0.01). Liuling Jiedu Wan could significantly reduce the capillary permeability caused by acetic acid and reduce the degree of ear swelling caused by xylene (P<0.05，P<0.01). The effects of Liuling Jiedu Wan on ear swelling in the middle and high dose groups were significantly better than that of Lanqin Oral Liquid. CONCLUSION Liuling Jiedu Wan has a good therapeutic effect on acute pharyngitis， and its mechanism may be related to the down-regulation of IL-1β， IL-6 and TNF-α levels. Under the clinical equivalent dose， the anti-inflammatory effect of Liuling Jiedu Wan is slightly better than that of Lanqin Oral Liquid， indicating that Liuling Jiedu Wan has a better effect on acute pharyngitis.