Application Progress and Prospect of Model Organism Zebrafish in Activity Screening and Toxicity Evaluation of Traditional Chinese Medicine

LI Yi; LIU Xia-jin; SU Shu-lan; YU Li; ZHU Yue; QIAN Da-wei; ZHAO Ming; DUAN Jin-ao

Volume 36 Issue 5

Sep. 2020

Turn off MathJax

Article Contents

Abstract

References

Journal of Nanjing University of traditional Chinese Medicine > 2020 > 36(5): 715-720.

LI Yi, LIU Xia-jin, SU Shu-lan, YU Li, ZHU Yue, QIAN Da-wei, ZHAO Ming, DUAN Jin-ao. Application Progress and Prospect of Model Organism Zebrafish in Activity Screening and Toxicity Evaluation of Traditional Chinese Medicine[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(5): 715-720.

Citation:

PDF (1462 KB)

Application Progress and Prospect of Model Organism Zebrafish in Activity Screening and Toxicity Evaluation of Traditional Chinese Medicine

More Information

Graphical Abstract

Abstract

Abstract

As a non-mammalian model organism， zebrafish has been widely used in the field of medical research because of its advantages of small size， easy feeding， rapid development， strong reproductive power， and easy observation. In recent years， with the continuous improvement of technology and methods， the application of model organism zebrafish in the field of traditional Chinese medicine (TCM) has also gradually deepened， especially for the evaluation and screening of complex active ingredients， active substances and toxicity of TCM. As a whole animal model， zebrafish can objectively evaluate the active substances and toxicity of TCM to achieve fast and effective high-throughput screening. In this article， by reviewing and summarizing the relevant literature at home and abroad in recent years， the application progress of model biological zebrafish in the screening of TCM activity and toxicity evaluation is reviewed， mainly from anti-inflammatory， anti-angiogenesis， pro-angiogenesis， anti-osteoporosis， Anti-alcoholic liver injury， anti-neurological disease and other active ingredients screening and hepatotoxicity and embryotoxicity are summarized and analyzed in order to provide new methods and techniques for the evaluation of the safety and effectiveness of TCM.
- zebrafish,
- model organism,
- traditional Chinese medicine,
- active screening,
- toxicity

FullText(HTML)

References (40)

References

[1]	孙智慧，贾顺姬，孟安明. 斑马鱼: 在生命科学中畅游[J]. 生命科学， 2006， 18(5): 431-436.
[2]	STREISINGER G， WALKER C， DOWER N， et al. Production of clones of homozygous diploid zebra fish (Brachydanio rerio)[J]. Nature， 1981， 291(5813): 293-296.
[3]	全珊珊，吴新荣. 斑马鱼，人类疾病研究的理想模式动物[J]. 生命的化学， 2008， 28(3): 260-263.
[4]	杨丽玲，余林中. 斑马鱼: 一种可用于中药抗炎免疫药理研究的模式生物[J]. 中药药理与临床， 2012， 28(2): 175-178.
[5]	GE H， TANG H， LIANG Y， et al. Rhein attenuates inflammation through inhibition of NF-κB and NALP3 inflammasome in vivo<\i> and in vitro<\i>[J]. Drug Des Devel Ther， 2017， 11: 1663-1671.
[6]	GUO DL， CHEN JF， TAN L， et al. Terpene glycosides from Sanguisorba officinalis<\i> and their anti-inflammatory effects[J]. Molecules， 2019， 24(16): 2906.
[7]	ZHOU H， CAO H， ZHENG Y， et al. Liang-Ge-San， a classic traditional Chinese medicine formula， attenuates acute inflammation in zebrafish and RAW 264.7 cells[J]. J Ethnopharmacol， 2020， 249: 112427.
[8]	ZHANG Y， TAKAGI N， YUAN B， et al. The protection of indolealkylamines from LPS-induced inflammation in zebrafish[J]. J Ethnopharmacol， 2019， 243: 112122.
[9]	GE H， TANG H， LIANG Y， et al. Rhein attenuates inflammation through inhibition of NF-κB and NALP3 inflammasome in vivo<\i> and in vitro<\i>[J]. Drug Des Devel Ther， 2017， 11: 1663-1671.
[10]	ISOGAI S， HORIGUCHI M， WEINSTEIN BM. The vascular anatomy of the developing zebrafish: An atlas of embryonic and early larval development[J]. Dev Biol， 2001， 230(2): 278-301.
[11]	NY A， AUTIERO M， CARMELIET P. Zebrafish and Xenopus<\i> tadpoles: Small animal models to study angiogenesis and lymphangiogenesis[J]. Exp Cell Res， 2006， 312(5): 684-693.
[12]	HU WH， CHAN GKL， LOU JS， et al. The extract of Polygoni Cuspidati Rhizoma et Radix<\i> suppresses the vascular endothelial growth factor-induced angiogenesis[J]. Phytomedicine， 2018， 42: 135-143.
[13]	何育霖，杨雨婷，何贝轩，等. 紫草素对斑马鱼胚胎毒性和血管抑制作用[J]. 中成药， 2016， 38(2): 241-245.
[14]	ALEX D， LAM IK， LIN Z， et al. Indirubin shows anti-angiogenic activity in an in vivo<\i> zebrafish model and an in vitro<\i> HUVEC model[J]. J Ethnopharmacol， 2010， 131(2): 242-247.
[15]	CHEN Y， CHEN PD， BAO BH， et al. Anti-thrombotic and pro-angiogenic effects of Rubia cordifolia<\i> extract in zebrafish[J]. J Ethnopharmacol， 2018， 219: 152-160.
[16]	LI J， ZHANG J， ZOU L， et al. Pro-angiogenic effects of Ilexsaponin A1 on human umbilical vein endothelial cells in vitro<\i> and zebrafish in vivo<\i>[J]. Phytomedicine， 2017， 36: 229-237.
[17]	ZHOU ZY， XIAO Y， ZHAO WR， et al. Pro-angiogenesis effect and transcriptome profile of Shuxinyin formula in zebrafish[J]. Phytomedicine， 2019， 65: 153083.
[18]	ZHANG JY， LIU MQ， HUANG MH， et al. Ginsenoside F1 promotes angiogenesis by activating the IGF-1/IGF1R pathway[J]. Pharmacol Res， 2019， 144: 292-305.
[19]	詹扬，韦英杰，孙娥，等. 基于斑马鱼模型的朝鲜淫羊藿抗骨质疏松活性部位筛选[J]. 中草药， 2014， 45(14): 2036-2041.
[20]	ZHANG S， ZHANG Q， ZHANG D， et al. Anti-osteoporosis activity of a novel Achyranthes bidentata<\i> polysaccharide via stimulating bone formation[J]. Carbohydr Polym， 2018， 184: 288-298.
[21]	LUO S， YANG Y， CHEN J， et al. Tanshinol stimulates bone formation and attenuates dexamethasone-induced inhibition of osteogenesis in larval zebrafish[J]. J Orthop Translat， 2016， 4: 35-45.
[22]	MILLER AM， HORIGUCHI N， JEONG WI， et al. Molecular mechanisms of alcoholic liver disease: Innate immunity and cytokines[J]. Alcohol Clin Exp Res， 2011， 35(5): 787-793.
[23]	GUO F， ZHENG K， BENED-UBIETO R， et al. The lieber-DeCarli diet-A flagship model for experimental alcoholic liver disease[J]. Alcohol Clin Exp Res， 2018， 42(10): 1828-1840.
[24]	HOWARTH DL， PASSERI M， SADLER KC. Drinks like a fish: Using zebrafish to understand alcoholic liver disease[J]. Alcohol Clin Exp Res， 2011， 35(5): 826-829.
[25]	LIN JN， CHANG LL， LAI CH， et al. Development of an animal model for alcoholic liver disease in zebrafish[J]. Zebrafish， 2015， 12(4): 271-280.
[26]	周振婷. 橙皮苷对斑马鱼酒精性脂肪肝的治疗作用及其机制研究[D]. 广州: 南方医科大学， 2017.
[27]	夏青，韩利文，张云，等. 基于斑马鱼模型的柴胡皂苷a保肝作用与肝毒性研究[J]. 中国中药杂志， 2019， 44(13): 2662-2666.
[28]	KALUEFF AV， GEBHARDT M， STEWART AM， et al. Towards a comprehensive catalog of zebrafish behavior 1.0 and beyond[J]. Zebrafish， 2013， 10(1): 70-86.
[29]	BUENAFE OE， ORELLANA-PAUCAR A， MAES J， et al. Tanshinone ⅡA exhibits anticonvulsant activity in zebrafish and mouse seizure models[J]. ACS Chem Neurosci， 2013， 4(11): 1479-1487.
[30]	ZHANG S， LIU X， SUN M， et al. Reversal of reserpine-induced depression and cognitive disorder in zebrafish by sertraline and Traditional Chinese Medicine (TCM)[J]. Behav Brain Funct， 2018， 14(1): 13.
[31]	夏婧，游秋云，黄攀攀，等. 中药酸枣仁对斑马鱼睡眠剥夺模型的行为学及神经递质受体的影响[J]. 时珍国医国药， 2019， 30(9): 2061-2064.
[32]	ZHAO C， JIA Z， LI E， et al. Hepatotoxicity evaluation of Euphorbia kansui<\i> on zebrafish larvae in vivo<\i>[J]. Phytomedicine， 2019， 62: 152959.
[33]	ZHAO C， WANG M， JIA Z， et al. Similar hepatotoxicity response induced by Rhizoma Paridis in zebrafish larvae， cell and rat[J]. J Ethnopharmacol， 2020， 250: 112440.
[34]	全云云，周忆梦，刘美辰，等. 斑马鱼模型筛选何首乌肝毒性的物质基础[J]. 中国实验方剂学杂志， 2019， 25(6): 52-57.
[35]	姚芳，张楷承，曹雨诞，等. 京大戟醋制前后对斑马鱼胚胎肝、胃肠毒性的影响[J]. 中国中药杂志， 2019， 44(6): 1179-1185.
[36]	段亚辉，张云，王雪，等. 基于模式生物斑马鱼的款冬叶肝肾毒性比较研究[J]. 中草药， 2019， 50(3): 669-674.
[37]	付晓春，沈小莉，俞航萍，等. 雷公藤多苷促进斑马鱼肝细胞凋亡的实验研究[J]. 中国医院药学杂志， 2019， 39(10): 1032-1038.
[38]	SUN W， YAN B， WANG R， et al. In vivo<\i> acute toxicity of detoxified Fuzi (lateral root of Aconitum carmichaeli<\i>) after a traditional detoxification process[J]. Excli J， 2018， 17: 889-899.
[39]	WANG T， WANG CX， WU Q， et al. Evaluation of tanshinone ⅡA developmental toxicity in zebrafish embryos[J]. Molecules， 2017， 22(4): 660.
[40]	LI J， ZHANG Y， LIU K， et al. Xiaoaiping induces developmental toxicity in zebrafish embryos through activation of ER stress， apoptosis and the wnt pathway[J]. Front Pharmacol， 2018， 9: 1250.

Cited By

Get Citation

PDF

XML

Article Metrics

Article views (580) PDF downloads (302)

Application Progress and Prospect of Model Organism Zebrafish in Activity Screening and Toxicity Evaluation of Traditional Chinese Medicine

Abstract

References

Catalog

Article Metrics

Related

Application Progress and Prospect of Model Organism Zebrafish in Activity Screening and Toxicity Evaluation of Traditional Chinese Medicine

Abstract

References

Catalog

Article Metrics

Related

Export File

Citation

Format

Content