Analyze and Verify the Potential Anti-Tumor Mechanism of Higenamine in Aconitum Carmichaeli Debx<\i> Based on Biological Databases

OBJECTIVE To explore the possible role and mechanism of Aconitum Carmichaeli Debx in the treatment of tumors， by using one of the main active components， higenamine as a representative. METHODS BATMAN-TCM and various biological databases were used to predict the possible mechanism of higenamine in regulating the occurrence and development of tumor， and the effects and mechanisms were verified by in vivo and in vitro experiments. RESULTS Database analysis showed that higenamine regulated the proliferation and apoptosis of tumor cells directly， or indirectly affected tumor occurrence and development by affecting heart function. The animal experiments showed that higenamine significantly reduced the volume of subcutaneous transplanted tumors of colon cancer (P<0.05)， promoted the apoptosis of tumor cells in tumor tissues， and significantly improved the secretion level of atrial natriuretic peptide (ANP) (P<0.01) of colon cancer model mice. However， the cell experiments found that higenamine had no direct killing effect on tumor cells. CONCLUSION Higenamine， one of the main active components of Aconitum Carmichaeli Debx， can inhibit the growth of colon cancer subcutaneously transplanted tumors and induce tumor cells apoptosis， playing an anti-tumor effect. The mechanism may be related to the promotion of the release of cardiac peptide hormone ANP. It can provide a certain theoretical basis for the clinical use of Aconitum Carmichaeli Debx to treat malignant tumors. However， the relationship between the toxicity of Aconitum Carmichaeli Debx and its anti-tumor effect remains to be further studied.