Preparation of Total Flavonoids from Stem and Leave of <i>Scutellaria Baicalensis<\i>

XUChao-qie; JIANGQun-yan; JIANGQi-hang; XUMing-ming; SUXiao-rong; ZENGJian-guo; DUANJin-ao; ZHAOMing

Volume 36 Issue 5

Sep. 2020

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Journal of Nanjing University of traditional Chinese Medicine > 2020 > 36(5): 593-599.

Citation: XUChao-qie, JIANGQun-yan, JIANGQi-hang, XUMing-ming, SUXiao-rong, ZENGJian-guo, DUANJin-ao, ZHAOMing. Preparation of Total Flavonoids from Stem and Leave of Scutellaria Baicalensis<\i>[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(5): 593-599.

XUChao-qie, JIANGQun-yan, JIANGQi-hang, XUMing-ming, SUXiao-rong, ZENGJian-guo, DUANJin-ao, ZHAOMing. Preparation of Total Flavonoids from Stem and Leave of Scutellaria Baicalensis<\i>[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(5): 593-599.

Citation: XUChao-qie, JIANGQun-yan, JIANGQi-hang, XUMing-ming, SUXiao-rong, ZENGJian-guo, DUANJin-ao, ZHAOMing. Preparation of Total Flavonoids from Stem and Leave of Scutellaria Baicalensis<\i>[J]. Journal of Nanjing University of traditional Chinese Medicine, 2020, 36(5): 593-599.

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Preparation of Total Flavonoids from Stem and Leave of Scutellaria Baicalensis<\i>

XUChao-qie¹,

JIANGQun-yan¹,

JIANGQi-hang¹,

XUMing-ming¹,

SUXiao-rong¹,

ZENGJian-guo²,

DUANJin-ao¹,

ZHAOMing¹

1.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Key Laboratory of Chinese Medicinal Resources Recycling Utilization under State Administration of Traditional Chinese Medicine, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China

2.
National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resource and Initiative, Hunan Key Laboratory of Traditional Chinese Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China

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Abstract

Abstract

OBJECTIVE To optimize the technology of preparing total flavonoids from the stem-leave of Scutellaria Baicalensis and provide basis for the rational utilization and development of stem-leave resources of Scutellaria Baicalensis. METHODS The transfer rates of 8 flavonoids compounds in the stem-leave of Scutellaria Baicalensis and the single factor combined with orthogonal design were applied to optimize the extraction process. In addition， the transfer rates of acid deposition and macroporous resin method to purify total flavonoids in the stem-leave were compared， and the purification technique of macroporous resin method was optimized. RESULTS The best extraction technology was to use 12 times amount of 40% ethanol with heat reflux， one time an hour. According to the result of purification of extraction， the transfer rate of total flavonoids in the stem-leave of Scutellaria Baicalensis with macroporous resin method was 92.53%， higher than that with acid deposition， 76.69%. When pH value was 1.01， with acid deposition， the transfer rate of compounds such as scutellarin was over 90%， while the transfer rate of enriched dihydroflavanone was quite low， for instance， 64.76% for isocyanin-7-O-β-D- glucuronide and 68.75% for safflower-7-O-β-D-glucuronide. However， as for macroporous resin method， the transfer rate could come up to over 90%， which promoted the efficient transfer of each target component. On the basis of optimum， the resource volume was 2.08 g dry extract per 10 g HP-20 type dry resin with 0.26 g/mL liquor. The ratio of resin column diameter to height was 1∶3. The perfect purification technology was to wash the sample with 4 BV of distilled water and eluted with 4 BV of 30% ethanol， which could obtain 66.03% mass fraction of total flavonoids. CONCLUSION This study establishes a firm and environmental-friendly method of preparing total flavonoids from the stem-leave of Scutellaria Baicalensis and provides some reference for the further development and utilization of the stem-leave of Scutellaria Baicalensis.

Keywords:
stem and leave of Scutellaria baicalensis<\i>,
total flavonoids,
extraction,
purification

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References (23)

References

[1]
国家药典委员会. 中华人民共和国药典：三部[M]. 北京: 中国医药科技出版社， 2016.

[2]
ZHAO Q， CHEN XY， MARTIN C. Scutellaria baicalensis<\i>， the golden herb from the garden of Chinese medicinal plants[J]. Sci Bull， 2016， 61(18): 1391-1398.

[3]
YAN B， XU W， SU S， et al. Comparative analysis of 15 chemical constituents in Scutellaria baicalensis<\i> stem-leaf from different regions in China by ultra-high performance liquid chromatography with triple quadrupole tandem mass spectrometry[J]. J Sep Sci， 2017， 40(18): 3570-3581.

[4]
王文涛，陈瑞，曹瑶，等. 中药黄芩资源研究进展[J]. 陕西农业科学， 2019， 65(4): 87-91.

[5]
ZHAO Q， ZHANG Y， WANG G， et al. A specialized flavone biosynthetic pathway has evolved in the medicinal plant，Scutellaria baicalensis<\i>[J]. Sci Adv， 2016， 2(4): e1501780.

[6]
LIU G， RAJESH N， WANG X， et al. Identification of flavonoids in the stems and leaves of Scutellaria baicalensis<\i> Georgi[J]. J Chromatogr B Analyt Technol Biomed Life Sci， 2011， 879(13/14): 1023-1028.

[7]
张琳.黄芩茎叶抑菌抗炎活性及抗小鼠乳腺炎作用的研究[D]. 南京:南京中医药大学， 2019.

[8]
杨志军，杨秀娟，李晶，等. 甘肃不同产地黄芩茎叶和根对急性肝损伤小鼠的影响[J]. 中国中医药信息杂志， 2015， 22(6): 61-63.

[9]
梅盛前，肖捷，李放，等. 黄芩茎叶黄酮对大鼠急性脊髓损伤后的神经保护作用及对HMGB1/TLR4/NF-κB信号通路的影响[J]. 中国中医急症， 2018， 27(5): 802-805， 816.

[10]
王秋杰，辛立升，吴晓光，等. 黄芩茎叶总黄酮对大鼠学习记忆的影响及抗氧化作用机制研究[J]. 河北医学， 2011， 17(11): 1434-1436.

[11]
佟继铭，陈光晖，刘玉玲，等. 黄芩茎叶总黄酮对家兔实验性动脉粥样硬化的预防作用[J]. 中草药， 2005， 36(1): 93-95.

[12]
史高峰，祝娟娟，张兴潜，等. 黄芩茎叶中总黄酮提取工艺优化[J]. 食品科学， 2012， 33(20): 109-112.

[13]
张慧，李媛，徐倩文，等. 黄芩茎叶中活性成分野黄芩苷的微波提取工艺[J]. 食品研究与开发， 2012， 33(8): 74-78.

[14]
骆冉冉，刘文娣，邓丽莉，等. 响应面法优化黄芩茎叶总黄酮提取工艺[J]. 食品安全质量检测学报， 2013， 4(6): 1865-1872.

[15]
朱磊，李君，董海荣，等. 黄芩茎叶提取物纯化方法优选[J]. 中国医药导报， 2014， 11(32): 90-93.

[16]
朱邦胜，孙洪胜，蔡明. 黄芩茎叶中野黄芩苷的提取工艺研究[J]. 中医药临床杂志， 2015， 27(5): 732-735.

[17]
李莉，周卫涛，李峰. 正交试验法优选黄芩茎叶中野黄芩苷提取工艺研究[J]. 世界最新医学信息文摘， 2017， 17(62): 108-109.

[18]
张雅蓉，徐犇，李纳，等. 响应面法优化黄芩茎叶对照提取物纯化工艺及其特征图谱研究[J]. 中国现代中药， 2019， 21(5): 654-661.

[19]
严宝飞. 黄芩茎叶资源化学与新资源药材质量评价研究[D]. 南京: 南京中医药大学， 2018.

[20]
金毅力. 药用植物黄芩(Scutellaria baicalensis<\i> Georgi)他感作用的初步研究[D]. 杭州: 浙江大学， 2010.

[21]
LI K， YAO F， XUE Q， et al. Inhibitory effects against α-glucosidase and α-amylase of the flavonoids-rich extract from Scutellaria baicalensis<\i> shoots and interpretation of structure-activity relationship of its eight flavonoids by a refined assign-score method[J]. Chem Cent J， 2018， 12(1): 82.

[22]
LI K， FAN H， YIN PP， et al. Structure-activity relationship of eight high content flavonoids analyzed with a preliminary assign-score method and their contribution to antioxidant ability of flavonoids-rich extract from Scutellaria baicalensis<\i> shoots[J]. Arab J Chem， 2018， 11(2): 159-170.

[23]
何春年，肖培根，彭勇，等. 一种含异红花素7-O-β<\i>-D-葡萄糖醛酸苷的活性提取物及其用途: 中国，CN102492008A[P]. 2012-06-13.

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[5] PANRong-hua, YANGChen, LIWei-dong. Study on Separation and Purification Technology of Yangshen-Paidu Fang by Macroporous Resin[J]. Journal of Nanjing University of traditional Chinese Medicine, 2015, 31(6): 593-597.

[6] Investigation on the Purification of Total Nucleoside and Nucleobases from Extraction in Ethanol Subsiding Method from Ruditapes Philippinarum[J]. Journal of Nanjing University of traditional Chinese Medicine, 2015, 31(3): 273-276.

[7] GAOYang, YIRuo-Kun, SONGJia-Le. Protective Effects of Total Flavonoids from Bamboo Leaves on UVB-irradiated Oxidative Damage in HaCaT Cells[J]. Journal of Nanjing University of traditional Chinese Medicine, 2015, 31(2): 165-169.

[8] HUJing-wen, WANGQiong, DILiu-qing. Study on Separation and Enriching Total Flavonoids and Total Alkaloids in Compound Gankang Tablet by Macroporous Adsorption Resin and Ion Exchange Resins[J]. Journal of Nanjing University of traditional Chinese Medicine, 2013, 29(3): 266-270.

[10] WU Ting, ZHANG Li. Determination of Content of Total Flavonoids in Gingko Wine with Absorption Spectrometry[J]. Journal of Nanjing University of traditional Chinese Medicine, 2005, 21(2): 129-130.

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[1]	国家药典委员会. 中华人民共和国药典：三部[M]. 北京: 中国医药科技出版社， 2016.
[2]	ZHAO Q， CHEN XY， MARTIN C. Scutellaria baicalensis<\i>， the golden herb from the garden of Chinese medicinal plants[J]. Sci Bull， 2016， 61(18): 1391-1398.
[3]	YAN B， XU W， SU S， et al. Comparative analysis of 15 chemical constituents in Scutellaria baicalensis<\i> stem-leaf from different regions in China by ultra-high performance liquid chromatography with triple quadrupole tandem mass spectrometry[J]. J Sep Sci， 2017， 40(18): 3570-3581.
[4]	王文涛，陈瑞，曹瑶，等. 中药黄芩资源研究进展[J]. 陕西农业科学， 2019， 65(4): 87-91.
[5]	ZHAO Q， ZHANG Y， WANG G， et al. A specialized flavone biosynthetic pathway has evolved in the medicinal plant，Scutellaria baicalensis<\i>[J]. Sci Adv， 2016， 2(4): e1501780.
[6]	LIU G， RAJESH N， WANG X， et al. Identification of flavonoids in the stems and leaves of Scutellaria baicalensis<\i> Georgi[J]. J Chromatogr B Analyt Technol Biomed Life Sci， 2011， 879(13/14): 1023-1028.
[7]	张琳.黄芩茎叶抑菌抗炎活性及抗小鼠乳腺炎作用的研究[D]. 南京:南京中医药大学， 2019.
[8]	杨志军，杨秀娟，李晶，等. 甘肃不同产地黄芩茎叶和根对急性肝损伤小鼠的影响[J]. 中国中医药信息杂志， 2015， 22(6): 61-63.
[9]	梅盛前，肖捷，李放，等. 黄芩茎叶黄酮对大鼠急性脊髓损伤后的神经保护作用及对HMGB1/TLR4/NF-κB信号通路的影响[J]. 中国中医急症， 2018， 27(5): 802-805， 816.
[10]	王秋杰，辛立升，吴晓光，等. 黄芩茎叶总黄酮对大鼠学习记忆的影响及抗氧化作用机制研究[J]. 河北医学， 2011， 17(11): 1434-1436.
[11]	佟继铭，陈光晖，刘玉玲，等. 黄芩茎叶总黄酮对家兔实验性动脉粥样硬化的预防作用[J]. 中草药， 2005， 36(1): 93-95.
[12]	史高峰，祝娟娟，张兴潜，等. 黄芩茎叶中总黄酮提取工艺优化[J]. 食品科学， 2012， 33(20): 109-112.
[13]	张慧，李媛，徐倩文，等. 黄芩茎叶中活性成分野黄芩苷的微波提取工艺[J]. 食品研究与开发， 2012， 33(8): 74-78.
[14]	骆冉冉，刘文娣，邓丽莉，等. 响应面法优化黄芩茎叶总黄酮提取工艺[J]. 食品安全质量检测学报， 2013， 4(6): 1865-1872.
[15]	朱磊，李君，董海荣，等. 黄芩茎叶提取物纯化方法优选[J]. 中国医药导报， 2014， 11(32): 90-93.
[16]	朱邦胜，孙洪胜，蔡明. 黄芩茎叶中野黄芩苷的提取工艺研究[J]. 中医药临床杂志， 2015， 27(5): 732-735.
[17]	李莉，周卫涛，李峰. 正交试验法优选黄芩茎叶中野黄芩苷提取工艺研究[J]. 世界最新医学信息文摘， 2017， 17(62): 108-109.
[18]	张雅蓉，徐犇，李纳，等. 响应面法优化黄芩茎叶对照提取物纯化工艺及其特征图谱研究[J]. 中国现代中药， 2019， 21(5): 654-661.
[19]	严宝飞. 黄芩茎叶资源化学与新资源药材质量评价研究[D]. 南京: 南京中医药大学， 2018.
[20]	金毅力. 药用植物黄芩(Scutellaria baicalensis<\i> Georgi)他感作用的初步研究[D]. 杭州: 浙江大学， 2010.
[21]	LI K， YAO F， XUE Q， et al. Inhibitory effects against α-glucosidase and α-amylase of the flavonoids-rich extract from Scutellaria baicalensis<\i> shoots and interpretation of structure-activity relationship of its eight flavonoids by a refined assign-score method[J]. Chem Cent J， 2018， 12(1): 82.
[22]	LI K， FAN H， YIN PP， et al. Structure-activity relationship of eight high content flavonoids analyzed with a preliminary assign-score method and their contribution to antioxidant ability of flavonoids-rich extract from Scutellaria baicalensis<\i> shoots[J]. Arab J Chem， 2018， 11(2): 159-170.
[23]	何春年，肖培根，彭勇，等. 一种含异红花素7-O-β<\i>-D-葡萄糖醛酸苷的活性提取物及其用途: 中国，CN102492008A[P]. 2012-06-13.

[1]	XIE Jia-yu, DAI Long-chao, WANG Na, LIU Cheng, WANG Ling-chong. Isolation and Purification of Alcohol-Extracted Protein from Eupolyphaga Sinensis Walker and Its in vitro Anti-Hepatocellular Carcinoma and Anti-Hepatic Fibrosis Activities[J]. Journal of Nanjing University of traditional Chinese Medicine, 2022, 38(12): 1116-1127. DOI: 10.14148/j.issn.1672-0482.2022.1116
[2]	YAN Jing-yang, JIANG Shu, LIU Pei, ZHU Ying, LI Hui-wei, DUAN Jin-ao. Study on Comprehensive Extraction Technology of Polyprenols and Flavonoids From Dregs of Ginkgo Folium After Water Extracting[J]. Journal of Nanjing University of traditional Chinese Medicine, 2017, 33(1): 104-108.
[3]	ZHANG Yi, WANG Wang, CAI Yin, WANG Min, GU Chun-yan, YANG Ye. Purification， Structural Characterization and Immunomodulatory and Antitumor Activities of a Polysaccharide Isolated from Momordica Charantia L.[J]. Journal of Nanjing University of traditional Chinese Medicine, 2017, 33(1): 33-39.
[4]	ZHOU Ping, XIE Chen-qiong, CHEN Jian-wei, LI Xiang. Comparison of Three Kinds of Flavonoids and Total Flavonoids Contents in Different Organs of Scutellaria Baicalensis Geogi and Its Callus[J]. Journal of Nanjing University of traditional Chinese Medicine, 2016, 32(2): 191-194.
[5]	PANRong-hua, YANGChen, LIWei-dong. Study on Separation and Purification Technology of Yangshen-Paidu Fang by Macroporous Resin[J]. Journal of Nanjing University of traditional Chinese Medicine, 2015, 31(6): 593-597.
[6]	Investigation on the Purification of Total Nucleoside and Nucleobases from Extraction in Ethanol Subsiding Method from Ruditapes Philippinarum[J]. Journal of Nanjing University of traditional Chinese Medicine, 2015, 31(3): 273-276.
[7]	GAOYang, YIRuo-Kun, SONGJia-Le. Protective Effects of Total Flavonoids from Bamboo Leaves on UVB-irradiated Oxidative Damage in HaCaT Cells[J]. Journal of Nanjing University of traditional Chinese Medicine, 2015, 31(2): 165-169.
[8]	HUJing-wen, WANGQiong, DILiu-qing. Study on Separation and Enriching Total Flavonoids and Total Alkaloids in Compound Gankang Tablet by Macroporous Adsorption Resin and Ion Exchange Resins[J]. Journal of Nanjing University of traditional Chinese Medicine, 2013, 29(3): 266-270.
[10]	WU Ting, ZHANG Li. Determination of Content of Total Flavonoids in Gingko Wine with Absorption Spectrometry[J]. Journal of Nanjing University of traditional Chinese Medicine, 2005, 21(2): 129-130.

1.	徐静，李晓亮，高宁，王宇，匡海学，梁华. 商陆提取物药理作用研究进展. 世界中医药. 2024(02): 272-278 .
2.	赵临丽，于洪跃，顾宁. 高血压合并阻塞性睡眠呼吸暂停低通气综合征中西医研究进展. 中西医结合心脑血管病杂志. 2023(21): 3966-3968 .
3.	孟香花，陈玉花，丽丽，毕力格，乌日汉，白梅荣. 基于16srDNA蒙药蜀葵花醇提物对水负荷模型大鼠利尿作用机制分析. 中国民族医药杂志. 2022(03): 50-54 .
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Preparation of Total Flavonoids from Stem and Leave of Scutellaria Baicalensis<\i>

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