Study on Antioxidation of Ganoderma Lucidum Polysaccharide

OBJECTIVE To study the antioxidant activity of Ganoderma lucidum polysaccharides(GLP)， and to provide scientific basis for its application of anti-aging cosmetics. METHODS Chemical method was used to evaluate the total reducing ability， hydroxy radical and DPPH free radical scavenging ability of GLP. The anti-lipid peroxidation ability of GLP was investigated with respect to the production of peroxid and malondialdehyde. The effects of GLP on the protective effect of H₂O₂-induced oxidative damage on HaCaT cells was investigated by in vitro cell experiments. The effects of lab-made GLP emulsion on superoxide dismutase (SOD) activity， malondialdehyde (MDA)， and hydroxyproline (Hyp) contents in the skin of D-galactose subacute mouse aging model were investigated in vivo. RESULTS The reducing ability and the ability to scavenge two radicals above were concentration-dependent. At the same time， it showed that GLP could inhibit the production of lipid peroxidation products. At 60 h， the peroxide produced by GLP liposomes were one-ninth of that of blank liposomes， and the production of malondialdehyde were one-third of it. Furthermore， GLP had a protective effect on H₂O₂ induced oxidatively damaged HaCaT cells (P<0.01)， and the protective effect was enhanced with increasing polysaccharide concentration. The results of animal experiments showed that the SOD activity in the skin was significantly higher than that in the model group (P<0.05) after applying low-dose homemade GLP emulsion to the skin of mice; when applied at high doses， the MDA content decreased significantly and the Hyp content increased significantly (P<0.05)， the antioxidant capacity of lab-made GLP emulsion is slightly better than the commercially available emulsion. CONCLUSION The antioxidative activity of GLP was evaluated at multiple levels， and GLP emulsions with anti-aging effects were initially prepared， which provided a research basis for the development of GLP anti-aging cosmetics.