Effects of Luteolin and Its Analogues Against Myocardial Ischemia/Reperfusion Injury， Lipid Metabolism and Apoptosis

OBJECTIVE To explore the multi-component and on MIRI model rat multi-target mechanism of Chinese medicine against MIRI， the protective effects of luteolin， kaempferol and luteoloside on rat MIRI， and the inhibition on lipid-metabolization-related enzymes 11β-HSD1， LPAATβ， and apoptosis-related enzymes PTEN， and SHP-2. METHEODS Using a Langendorff apparatus， the MIRI model was established by stopping perfusion and reperfusion with oxygenated Locke-Ringer's solution. The HR， LVEDP， LVDP， (-dp/dt)/(+dp/dt)， CF， LDH and myocardial infarct size were assayed. LPAATβ and 11β-HSD1 were assayed by spectrophotometry. The inhibition of PTEN and SHP-2 activity was measured by spectrophotometry. RESULTS Luteolin， kaempferol and luteoloside could protect HR， reduce LDH release， raise LVDP， LVEDP and (-dp/dt)/ (+dp/dt)， recover CF， and reduce myocardial infarct size， respectively. Moreover， the activities of LPAATβ， 11β-HSD1， PTEN and SHP-2 were repressed by pretreatment with luteolin， kaempferol and luteoloside. CONCLUSION Luteolin， kaempferol and luteoloside show the cardioprotective effects on MIRI of isolated rat heart by against lipid metabolism disorders and apoptosis.