Quality Evaluation of Angelica Pubescens Radix Pieces by HPLC Fingerprint and Multi-Component Quantitative Analysis

OBJECTIVE To establish the HPLC fingerprint and simultaneous method for the determination of 6 components for processed pieces and quality control of Angelica Pubescens Radix pieces . METHODS Fingerprints of 15 batches of Angelica Pubescens Radix pieces were determined by HPLC-PDA， the control fingerprint was established. Combined with cluster analysis and partial least square discriminant analysis， the quality of 15 batches of Angelica Pubescens Radix pieces was analyzed. At the same time， the contents of columbianetin， angelol A， angelol G， columbianetin acetate， osthole， isoimperatorin and columbianadin were determined. RESULTS The similarity of 15 batches of Angelica Pubescens Radix pieces was 0.858-0.986， and 21 common peaks were identified. Columbianetin， angelol A， angelol G， columbianetin acetate， osthole， isoimperatorin and columbianadin were identified， among which columbianetin， angelol A， angelol G， columbianetin acetate， osthole， isoimperatorin and columbianadin were quantitative analyzed. Their contents were 0.013%-0.051%， 0.553%-0.866%， 0.222%-0.396%， 0.032%-0.259%， 0.327%-1.229%， 0.108%-0.473%， respectively. Cluster analysis divides 15 batches of decoction pieces into three categories， and partial least squares discriminant analysis marks 8 different components in the decoction pieces. CONCLUSION The fingerprint and multi-component quantitative analysis method established in this experiment are stable and reliable， which can provide theoretical guidance for the establishment of quality standards of Angelica Pubescens Radix pieces.