Study on the Cold-Heat Nature of Multicomponents in Fructus Evodiae Based on the Ethology of Animal Temperature Tropism

OBJECTIVE To expound the cold and heat properties of different components in Evodia rutaecarpa. METHODS KM mice were orally administrated with Evodia rutaecarpa water extract or its different elution parts. The different elution parts on the temperature tropism of mice， the ATP enzyme activity in mice liver， and the energy metabolism in mice were carried out to evaluate their cold-heat nature. RESULTS Compared with the blank control group， the proportion of mice staying in the low temperature area was significantly increased under the treatment of 60% ethanol washed part， 80% ethanol washed part and Evodia rutaecarpa aqueous extract group (P<0.05). The retention rate of mice in the high temperature area was significantly higher in the washing group (P<0.05). The activities of Na-K-ATPase and Mg-ATPase in mice liver were significantly decreased (P<0.05) under the treatment of aqueous washing group. The activities of Ca-ATPase in mice liver of 20% group were significantly increased (P<0.05). The activity of Na-K-ATPase in mice liver of 60% and 80% group was higher than control group (P<0.05). The activity of Na-K-ATPase and Ca-ATPase of the Evodiae water extract group was higher than control group (P<0.05). Compared with the blank group， Evodia rutaecarpa water extract group and its 20%， 60% and 80% groups increased the mice oxygen consumption， heat production and energy metabolism (P<0.05). Evodia rutaecarpa water elution site reduced the consumption Oxygen， caloric and energy metabolism (P<0.05). CONCLUSION The thermal properties of Evodia rutaecarpa are the synthesis or addition of its cold and thermal components. Meanwhile， the thermal components resisted the cold nature of cold components and finally showed the nature of heat.