Mechanical Research of Chronic Oxidative Stress on the Activity of Breast Cancer Cells MCF-7 by Activating GSK3β/β-catenin Signaling Pathway

OBJECTIVE To establish a oxidative stress cell line model of breast cancer cells MCF-7， observe the growth under this environment， and explore the effects of chronic stress status on breast cancer proliferation， migration and invasion of breast cancer and its possible mechanisms. METHODS The CCK-8 assay was used to detect the proliferation inhibition rate of MCF-7 under the conditions of acute oxidative stress and chronic oxidative stress. The ability of MCF-7 cells to migrate and invade under chronic oxidative stress conditions was examined by scratch assay and transwell assay. The level of IL-6 was determined by ELISA. Superoxide dismutase activity (SOD) and total antioxidant capacity (T-AOC) of MCF-7 under chronic oxidative stress model conditions were tested by kit. The expression levels of GSK3β/β-catenin signaling pathway-related proteins and RNA were determined by RT-PCR and Western blot. RESULTS Compared with acute oxidative stress， under the condition of chronic oxidative stress， the value-adding ability of MCF-7 was enhanced， and the ability of SOD and T-AOC was further enhanced. Its ability to migrate and invade is stronger than that of wild-type and MCF-7 under acute oxidative stress. This result is consistent with IL-6 protein expression， GSK3β/β-catenin signaling pathway-associated protein and RNA expression. It was also found that Sanhuang Decoction can inhibit the proliferation of MCF-7 chronic oxidative stress cells. CONCLUSION Chronic oxidative stress can promote the proliferation and metastasis of breast cancer cells MCF-7， and its mechanism may be related to the activation of GSK3β/β-catenin signaling pathway and the increase of IL-6 expression.