Study on HPLC Fingerprint and Content Determination of Major Costituents of GJ-4，an Effective Fraction from Gardeniae Fructus

OBJECTIVE To establish a HPLC fingerprint of GJ-4 (an effective fraction from Gardeniae Fructus) and to quantify the main component crocin-3. METHODS The experiment was carried out on a Phenomenex Gemini C₁₈ column (250 mm×4.6 mm， 5 μm)， eluted with ACN-water with 1‰ formic gradiently and determined at 440 nm wavelength. HPLC fingerprint chromatogram of GJ-4 was set up and quality of 20 batches of GJ-4 was evaluated by similarity assay. The major contents of crocin-3 was determined. RESULTS The HPLC fingerprint chromatogram of GJ-4 was been established， in which 17 compounds were identified. The similarity of 20 batches of GJ-4 was about 0.939 to 0.994， and the content of crocin-3 was about 0.200 to 0.682 mg/g. CONCLUSION The method established in this paper can provide a comprehensive reference for the quality control of GJ-4.