Volume 34 Issue 4
Jul.  2018
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Study on HPLC Fingerprint and Content Determination of Major Costituents of GJ-4，an Effective Fraction from Gardeniae Fructus[J]. Journal of Nanjing University of traditional Chinese Medicine, 2018, 34(4): 416-420.
Citation: Study on HPLC Fingerprint and Content Determination of Major Costituents of GJ-4，an Effective Fraction from Gardeniae Fructus[J]. Journal of Nanjing University of traditional Chinese Medicine, 2018, 34(4): 416-420.
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Study on HPLC Fingerprint and Content Determination of Major Costituents of GJ-4，an Effective Fraction from Gardeniae Fructus

  • 1.

    Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou, 510632, China

  • 2.

    Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drug Research, Guangzhou, 510632, China

  • 3.

    Pharmaceutical Institute of the Academy of Medical Science of China, State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing, 100050, China

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    • Abstract
  • OBJECTIVE To establish a HPLC fingerprint of GJ-4 (an effective fraction from Gardeniae Fructus) and to quantify the main component crocin-3. METHODS The experiment was carried out on a Phenomenex Gemini C18 column (250 mm×4.6 mm， 5 μm)， eluted with ACN-water with 1‰ formic gradiently and determined at 440 nm wavelength. HPLC fingerprint chromatogram of GJ-4 was set up and quality of 20 batches of GJ-4 was evaluated by similarity assay. The major contents of crocin-3 was determined. RESULTS The HPLC fingerprint chromatogram of GJ-4 was been established， in which 17 compounds were identified. The similarity of 20 batches of GJ-4 was about 0.939 to 0.994， and the content of crocin-3 was about 0.200 to 0.682 mg/g. CONCLUSION The method established in this paper can provide a comprehensive reference for the quality control of GJ-4.
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    • References (19)
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