Study on the Anti-Hyperglycemic Activity and Polysaccharide and Protein Components in Different Alcohol Precipitate Fraction of Mactra Veneriformis

OBJECTIVE To screen the effective anti-hyperglycemic fraction of Mactra veneriformis， determine and identify polysaccharide and protein components in different alcohol precipitate fraction of Mactra veneriformis. METHODS With the blood glucose concentration of mice with alloxan-induced diabetes mellitus as screening index， then the blood was taken by removalling eyeball and INS， TG， T-CHO were determined; the content of polysaccharide and protein were determined by the anthrone-sulfuric and BCA method； the proteins were checked through SDS-polyacrylamide gel electrophoresis and LC-MS/MS. RESULTS Except 20% alcohol precipitate fraction， the others reduced the content of mice blood sugar， INS and TG. 80% alcohol precipitate fraction reduced T-CHO; the content of polysaccharide and protein in 20%， 40%， 60%， 80% alcohol precipitate fraction respectively were 21.45%， 45.82%， 14.61%， 22.84% and 23.56%， 16.85%， 28.60%， 36.10%；the kinds of protein included myosin， tropomyosin， actin， calmodulin， elastin and tubulin. CONCLUSION 60% and 80% alcohol precipitate fraction shows remarkable effects on reducing mice blood sugar content， which can be further developed as a hypoglycemic product.