Study on the Intervention Effect of Shaofu Zhuyu Decoction on Blood Stasis Syndrome Based upon Non-target Plasma Metabolomics Strategy

OBJECTIVE To explore the therapeutic mechanism of Shaofu Zhuyu decoction（SFZYD） on blood stasis syndrome from the perspective of plasma metabolomics. METHODS A blood stasis syndrome rats model was established by ice-water bath in combination with an injection with adrenaline. The plasma metabolomic profile was obtained by gas chromatography coupled with mass spectrometry (GC-MS). Fold change and one-way analysis of variance (ANOVA) were used to search for potential biomarkers. RESULTS Finally， eight potential biomarkers， such as asparagine， glutamic acid， ribitol and uracil， were obtained. The levels of these potential biomarkers， except that of cystine， were decreased significantly in blood stasis syndrome rats. SFZYD at low dose significantly increased the level of uracil， and reversed other potential biomarkers， except 3-phosphoglycerate， into normal level; SFZYD at high dose significantly adjusted the levels of asparagine and ribitol， and xylitol and uracil showed trend towards the normal levels. CONCLUSION SFZYD shows good therapeutic effect on blood stasis syndrome by regulating the metabolism of amino acids and energy， and different doses of SFZYD may have different regulating effects on the potential biomarkers associated with blood stasis.