Effect of Bran Stir-Baked Atractylodis Rhizoma Water Extract， Ethanol Extract and CH2Cl2 Active Parts on Spleen-Deficiency Diarrhea in Mice

OBJECTIVE Study of bran stir-baked Atractylodis Rhizoma water extract，ethanol extract and CH2Cl2 active parts on Efficacy with Spleen-deficiency Diarrhea(SDD) in mice. METHODS The spleen deficiency mouse models were established by extract of Sennae Folium and treated by bran stir-baked Atractylodis Rhizoma water extract， ethanol extract and CH2Cl2 active parts.Body weight，diarrhea index， fecal moisture，levels of serum amylase，D-xylose in serum and short chain fatty acids (SCFAs) contents in cecum of mice were studied， and intestinal pathology examination were observed. RESULTS Compared with model group， the body weights， the levels of D-xylose and SCFAs contents in the water extract， ethanol extract and CH2Cl2 active parts from bran stir-baked Atractylodis Rhizoma obviously increased. but the levels of diarrhea index， fecal moisture and relieve intestinal mucosal injury were significantly decreased. moreover，the level of serum amylase in the CH2Cl2 active parts group increased compared with model group. CONCLUSION The water extract，ethanol extract and CH2Cl2 active parts from bran stir-baked Atractylodis Rhizoma have obvious anti diarrhea effect in mice caused by extract of Sennae Folium. CH2Cl2 active parts show best improvement in digestion and absorption function.