Urine Metabonomic Study on Xiaofeng Xuanqiao Decoction in Treatment of Anaphylactic Rhinitis Based on UPLC/LTQ-Orbitrap-MS

OBJECTIVE To observe the regulation of Xiaofeng Xuanqiao Decoction(XFTQ) on abnormal metabolites by analyzing the changes of endogenous metabolites in urine of anaphylactic rhinitis(AR) mice， and to explore the mechanism of Xiaofeng Xuanqiao Decoction on treating AR from the perspective of metabonomics. METHODS 60 BALB/c mice were divided into normal group， model group， montelukas group and XFTQ group. Intraperitoneal injection of OVA and local nasal mucosa stimulation was used to establish AR model. Montelukast group and XFTQ group were given montelukast sodium tablets and Xiaofeng Xuanqiao decoction， respectively， for 7d. Urine samples were collected from each group on the last day of administration. UPLC/LTQ-Orbitrap-MS was applied to analyze the metabolic profile changes of each group and the intervention mechanism of drugs. RESULTS AR disordered the metabolism of acid， amine and others in mice. Xiaofeng Decoction mainly regulated the levels of 14 kinds of abnormal metabolites， and it showed good intervention effect on AR mice. CONCLUSION Small molecule metabolites in AR mice are deviated from the normal group. Xiaofeng Xuanqiao Decoction improves the histidine metabolism and cysteine and methionine metabolism to achieve therapeutic effect， mainly through the up-regulation of asymmetric dimethyl-arginine（ADMA）， N-methylnicotinic acid， S-adenosyl-L-methionine(SAMe)， indole-2-carboxylic acid(I2CA)， 6-aminocaproic acid(EACA)， imidazole-4-acetic acid，1-methyl-4-pyridone-5-carboxamide(M4PY)， and the down-regulation of 1-methylhistamine.